PE anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104
BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE
  • 104
    BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE
Compare all formats See PE spectral data
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109807 50 µg 49€
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109808 200 µg 172€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  2. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  3. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  4. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  5. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  6. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  7. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  8. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  9. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  10. Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
  11. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  12. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  13. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  14. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  15. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  16. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  17. Davila S, et al. 2014. J Immunol. 192:4284. PubMed
  18. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  19. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  20. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  21. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  22. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  23. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  24. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  25. Amor C, et al. 2020. Nature. 583:127. PubMed
  26. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  27. He L, et al. 2019. iScience. 0.790972222. PubMed
  28. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  29. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
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  33. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  34. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  35. Chen D, et al. 2021. Molecular Cell. :. PubMed
  36. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  37. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  38. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  39. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  40. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  41. Ndeupen S, et al. 2022. STAR Protoc. 3:101350. PubMed
  42. Zhang X, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  43. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  44. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  45. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  46. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  47. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  48. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  49. Hirata T, et al. 2012. Int Immunol. 24:705. PubMed
  50. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  51. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  52. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  53. Xiong J, et al. 2011. Int Immunol. 23:56. PubMed
  54. Wang X, et al. 2021. Cell. 184:5357. PubMed
  55. Koide S, et al. 2022. iScience. 25:103603. PubMed
  56. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  57. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  58. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  59. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  60. Wang X, et al. 2022. Elife. 11:. PubMed
  61. Sindrilaru A, et al. 2009. Blood. 113:5266. PubMed
  62. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  63. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  64. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  65. Spath S, et al. 2022. iScience. 25:104998. PubMed
  66. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  67. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  68. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  69. Huang X, et al. 2021. Immunity. . PubMed
  70. Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
  71. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  72. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  73. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  74. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  75. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
  76. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  77. Young K, et al. 2021. Cell Stem Cell. . PubMed
  78. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
RRID
AB_313444 (BioLegend Cat. No. 109807)
AB_313445 (BioLegend Cat. No. 109808)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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