APC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_APC_090507
SJL mouse splenocytes stained with A20 APC
  • A20_APC_090507
    SJL mouse splenocytes stained with A20 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
110713 25 µg 32€
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110714 100 µg 92€
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  2. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  3. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  4. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  5. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  6. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  7. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  8. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  9. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  10. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  11. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  12. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  13. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  14. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  15. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  16. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  17. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  18. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  19. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  20. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  21. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  22. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  23. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  24. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  25. Ocaña-Morgner C, et al. 2013. J Immunol. 190:5545. PubMed
  26. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  27. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  28. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  29. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  30. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  31. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  32. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  33. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
RRID
AB_313502 (BioLegend Cat. No. 110713)
AB_313503 (BioLegend Cat. No. 110714)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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