Veri-Cells™...

Are most appropriate when you need reference/control cells for longitudinal, multi-center studies.

Products

Reference laboratories, clinical research organizations, and multi-center clinical trials, among other institutions, need reliable controls to monitor assay performance and variability for longitudinal studies. Veri-Cells™ is our line of lyophilized control cell products, which can be used to help monitor data quality and aid in reproducibility in multi-center and longitudinal studies. 

Veri-Cells™ feature:

  • Exceptional long term stability
  • Scatter profile similar to fresh samples
  • Validation for the detection of over 150 markers

For more helpful information on Veri-Cells™, visit the FAQ page.

 

Veri-Cells™ Activated (Cytokine) PBMC

Veri-Cells™ Activated (Cytokine) PBMC have been activated with our Cell Activation Cocktail (with Brefeldin A), which includes phorbol 12-myristate 13-acetate (PMA) and Ionomycin, for 6 hours. They have been validated to stain for activation-induced cytokines including IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN-γ, and TNF-α, along with surface markers typically expressed on human PBMCs. Cells do not require additional fixation nor permeabilization prior to cytokine staining, thus saving more time in your experiment.

 

 


 

Veri-Cells™ Activated (Surface)

Veri-Cells™ Activated (Surface) have been activated with Concanavalin A for 72 hours. This makes them ideal for the detection of T cell activation markers, including CD25, CD69, CD134, CD279, CD366, and TIGIT.

 

 


 

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway)

Veri-Cells™ Phospho PBMC are stimulated with PMA/Ionomycin for 15 minutes. This product includes both stimulated and vehicle control cells for the staining of RAS mitogen-activated kinase pathways, including phospho-S6, phospho-p38, and phospho-ERK1/2.

 

 


 

Veri-Cells™ CD34 PBMC

Veri-Cells™ CD34 PBMC contains mixture of CD34+ KG-1a cells and human PBMCs to help verify CD34 antibody binding without the need for artificial enrichment or the collection of a large number of events.

 

 


 

Veri-Cells™ Leukocytes 

Unlike Veri-Cells™ PBMC and CD4-Low PBMC, Veri-Cells™ Leukocytes contain neutrophils and eosinophil populations. These cells can be used as controls or reference material to monitor expression of granulocyte markers such as CD15 and CD16.

 

 


 

Veri-Cells™ CD4-Low PBMC

Veri-Cells™ CD4-Low PBMC are specially formulated to contain a lower frequency of CD4+ cells, similar to that observed in patients with CD4 immunodeficiency. The expected frequency of CD3+ CD4+ cells can be found on the certificate of analysis for that lot.

 

 


 

Veri-Cells™ PBMC


Veri-Cells™ PBMC consist of lyophilized human PBMCs validated for over 150 surface markers and intracellular targets and exhibit a scatter profile similar to freshly isolated cells. Please refer to the Validation Tab to view the full listing of markers and clones that can be used with Veri-Cells™ PBMC.

Stability

Veri-Cells™ are highly stable, whether used immediately, 5 days after reconstitution, or up to a year in its lyophilized state, making it perfect for long-term longitudinal studies.

Consistent cell frequences pre and post-lyophilization

 

Fresh PBMCs and reconstituted Veri-Cells™ PBMC were surface stained with anti-human CD3, CD19 and CD56/CD16 antibodies. The percentage of positive cells was similar before and after the lyophilization process.

Stability after reconstitution

 

Veri-Cells™ PBMC were surface stained with anti-human CD4 PE/Cy7, CD8 APC, CD19 PerCP/Cy5.5, CD56 FITC and CCR6 BV421™ on day 0 and day 5 post-reconstitution.

Long-term stability

 

Frequencies of CD3, CD4, CD8 positive T cells, and CD19 positive B cells were almost identical between Veri-Cells™ PBMC tested immediately post lyophilization and lyophilized Veri-Cells™ PBMC stored at room temperature for 18 months.

Excellent Surface Staining

Excellent Surface Staining

Transcription Factor Staining

Transcription Factor Staining

150+ Surface Markers Validated on Veri-Cells™ PBMC

The following cell surface markers were validated using BioLegend's LEGENDScreen™ Human PE kit.

 

 

Marker Clone
CD1c L161
CD1d 51.1
CD2 RPA-2.10
CD3 HIT3a
CD4 RPA-T4
CD5 UCHT2
CD6 BL-CD6
CD7 CD7-6B7
CD8a HIT8a
CD9 HI9a
CD11a HI111
CD11b ICRF44
CD11b CBRM1/5
CD11c 3.9
CD13 WM15
CD14 M5E2
CD16 3G8
CD18 TS1/18
CD19 HIB19
CD20 2H7
CD21 Bu32
CD22 HIB22
CD23 EBVCS-5
CD24 ML5
CD26 BA5b
CD27 O323
CD28 CD28.2
CD29 TS2/16
CD31 WM59
CD32 FUN-2
CD33 WM53
CD35 E11
CD36 5-271
CD38 HIT2
CD39 A1
CD40 HB14
CD41 HIP8
CD42b HIP1
CD43 CD43-10G7
CD44 BJ18
Marker Clone
CD45 HI30
CD45RA HI100
CD45RB MEM-55
CD45RO UCHL1
CD46 TRA-2-10
CD47 CC2C6
CD48 BJ40
CD49d 9F10
CD49e NKI-SAM-1
CD49f GoH3
CD50 CBR-IC3/1
CD52 HI186
CD53 HI29
CD54 HA58
CD55 JS11
CD56 HCD56
CD57 HCD57
CD58 TS2/9
CD59 p282 (H19)
CD61 VI-PL2
CD63 H5C6
CD64 10.1
CD69 FN50
CD73 AD2
CD74 LN2
CD79b CB3-1
CD81 5A6
CD82 ASL-24
CD84 CD84.1.21
CD85 MKT5.1
CD85d 42D1
CD85 GHI/75
CD86 IT2.2
CD87 VIM5
CD88 S5/1
CD89 A59
CD93 VIMD2
CD94 DX22
CD95 DX2
CD97 VIM3b
Marker Clone
CD99 HCD99
CD100 A8
CD101 BB27
CD102 CBR-IC2/2
CD116 4H1
CD122 TU27
CD123 6H6
CD124 G077F6
CD126 UV4
CD127 A019D5
CD132 TUGh4
CD134 Ber-ACT35 (ACT35)
CD148 A3
CD154 24-31
CD156c SHM14
CD158a/h HP-MA4
CD158b DX27
CD158e1 DX9
CD161 HP-3G10
CD162 KPL-1
CD163 GHI/61
CD164 67D2
CD165 SN2 (N6-D11)
CD172a SE5A5
CD172b B4B6
CD172g LSB2.20
CD180 MHR73-11
CD182 5E8/CXCR2
CD196 G034E3
CD197 G043H7
CD200 OX-104
CD200R OX-108
CD226 11A8
CD229 Hly-9.1.25
CD244 C1.7
CD268 11C1
CD272 MIH26
CD277 BT3.1
CD278 C398.4A
Marker Clone
CD279 EH12.2H7
CD284 HTA125
CD290 3C10C5
CD298 LNH-94
CD300e UP-H2
CD300F UP-D2
CD314 1D11
CD319 162.1
CD328 6-434
CD335 9E2
CD337 P30-15
CD352 NT-7
CD354 TREM-26
β2-microglobulin 2M2
C3aR hC3aRZ8
CLEC12A 50C1
CX3CR1 2A9-1
FcRL6 2H3
HLA-A,B,C W6/32
HLA-A2 BB7.2
HLA-DQ HLADQ1
HLA-DR L243
HLA-E 3D12
HVEM 122
IgD IA6-2
IgM MHM-88
Integrin β7 FIB504
NKp80 5D12
Siglec-9 K8
TCR gamma/delta B1
TCR Vβ13.2 H132
TCR Vβ23 αHUT7
TCR α/β IP26
TCR Vβ8 JR2 (JR.2)
TCR Vβ9 MKB1
TCR Vδ2 B6
TCR Vγ9 B3
TCR Vα7.2 3C10

100+ Surface Markers Validated on Veri-Cells™ Leukocytes

The following cell surface markers were validated using LEGENDScreen™ Human PE kit.

 

Marker Clone
β2-microglobulin 2M2
CD1c L161
CD1d 51.1
CD2 RPA-2.10
CD3 HIT3a
CD4 RPA-T4
CD5 UCHT2
CD6 BL-CD6
CD10 HI10a
CD11a HI111
CD11b ICRF44
CD13 WM15
CD14 M5E2
CD15 W6D3
CD15 (SSEA-1) MC-480
CD16 3G8
CD18 TS1/18
CD19 HIB19
CD20 2H7
CD21 Bu32
CD22 HIB22
CD23 EBVCS-5
CD24 ML5
CD26 BA5b
CD27 O323
CD28 CD28.2
CD29 TS2/16
CD31 WM59
CD32 Fun-2
CD33 WM53
Marker Clone
CD35 E11
CD38 HIT2
CD39 A1
CD40 HB14
CD43 CD43-10G7
CD44 BJ18
CD45 HI30
CD45RA HI100
CD45RB MEM-55
CD45RO UCHL1
CD47 CC2C6
CD48 BJ40
CD49d 9F10
CD49e NKI-SAM-1
CD50 CBR-IC3/1
CD52 HI186
CD53 HI29
CD55 JS11
CD56 HCD56
CD57 HCD57
CD58 TS2/9
CD59 p282 (H19)
CD64 10.1
CD66a/c/e ASL-32
CD66b G10F5
CD7 CD7-6B7
CD74 LN2
CD81 5A6
CD82 ASL-24
CD84 CD84.1.21
Marker Clone
CD85j GHI/75
CD86 IT2.2
CD87 VIM5
CD88 S5/1
CD89 A59
CD8a HIT8a
CD94 DX22
CD95 DX2
CD97 VIM3b
CD99 HCD99
CD101 BB27
CD102 CBR-IC2/2
CD116 4H1
CD122 TU27
CD127 A019D5
CD132 TUGh4
CD141 M80
CD155 SKII.4
CD156c SHM14
CD161 HP-3G10
CD163 GHI/61
CD164 67D2
CD172a/b SE5A5
CD172b B4B6
CD172g LSB2.20
CD180 MHR73-11
CD197 G043H7
CD200 OX-104
CD226 11A8
CD244 C1.7
Marker Clone
CD245 DY12
CD263 DJR3
CD268 11C1
CD278 C398.4A
CD279 (PD-1) EH12.2H7
CD284 HTA125
CD298 LNH-94
CD300e UP-H2
CD300F UP-D2
CD314 1D11
CD319 162.1
CD328 6-434
CD335 9E2
CD337 P30-15
CD354 TREM-26
CLEC12A 50C1
CX3CR1 2A9-1
DcR1 (TRAIL-R3) DJR3
Galectin-3 (Mac-2) Gal397
HLA-A,B,C W6/32
HLA-DQ HLADQ1
HLA-DR L243
IgD IA6-2
LTβR 31G4D8
NKp80 5D12
Siglec-9 K8
TCR α/β IP26
TCR gamma/delta B1
TCR Vδ2 B6
Vγ9 B3