LEGENDScreen™ Human PE Kit

Pricing & Availability
Other Names
Human Cell Surface Marker Screening (PE) Kit, Human Antibody Panel (PE)
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Product Citations
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Cat # Size Price Quantity Avail. Save
700007 1 kit (371 antibodies, 1 test per antibody) 1.200€
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Description

BioLegend's LegendScreen™ Human PE Kit contains 361 PE-conjugated monoclonal antibodies to cell surface markers as well as 10 mouse, rat, or hamster Ig isotype controls. All of the antibodies have been pre-titrated to optimal concentrations, arrayed on four 96-well plates, and lyophilized in a ready-to-use format. The kit can be used for screening human leukocytes, cell lines and cells isolated from tissues. There are blank wells on the plates for users to add their choice of markers. The end user can also choose to screen cells of interest directly or co-stain with other markers labeled with different fluorochromes. Positive "hits" from the screening can be quickly identified based on the plate map, and the catalog number. Clone information can be obtained using the tables in the product manual. Analysis with individual fluorochrome-conjugated antibodies should be performed to confirm the screening results.

The LEGENDScreen™ Human PE Kit provides a convenient, easy to use, and powerful tool for immunology, stem cell, and cancer research.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Application

Cell Screening

Recommended Usage

Instructions for use are detailed in the manual provided with each kit.

Application Notes

Materials Provided in Kit:

  • Four Human Cell Screening Plates containing:
    - 361 PE-conjugated Monoclonal Antibodies
    - 10 Mouse, Rat or Hamster Ig Isotype Controls
  • Cell Staining Buffer (Cat. No. 420201)
  • Fixation Buffer (Cat. No. 420801)
  • Plate Sealers (12 sheets)
  • Manual
Additional Product Notes

View more applications data using a previous version of this product to stain human PBMCs and to screen Veri-Cells™ lyophilized control cells in our Scientific Poster Library.
 

Download the Excel Version of the Specificity/Clone List

Product Citations
  1. Bojic S, et al. 2018. Stem Cells. 36:1723. PubMed
  2. Vattulainen M, et al. 2019. Stem Cell Res Ther. 10:236. PubMed
  3. Adkar SS, et al. 2019. Stem Cells. 37:65. PubMed
Publication Library

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

Are these plates made under sterile conditions?

The plates are not sterile. You would handle them as you would handle most typical flow cytometry staining protocols.

Can I use half or less of the plate and keep the rest for later?

Yes. Customers can use half of the plate or whatever specificities they are interested in. However, the whole plate should be reconstituted. The half plate of antibodies must be transferred to another empty plate for the staining. The remaining half must be sealed and stored at 2 - 8°C in the dark and used within a month.

Can I use my existing institution discount for this new product?

Yes.

How do I request a custom LEGENDScreen™ product with only my specificities of interest?

For more info, visit: biolegend.com/custom_solutions.

How should the kit be stored?

The kit should be stored at 2 - 8°C upon receipt. Once opened, the plates must be reconstituted immediately. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month.

I am not going to use all the reconstituted antibody solution. Can I keep the left over for later or re-dry the solution in dark?

The antibody is in a one test per well format. There will not be any antibody left if the full test is used. Customers may decide to use less than the recommended volume per test, but this is not recommended and the performance is not guaranteed. Customers may also selectively transfer certain antibodies from the original plate to a new plate and use after reconstitution. If any antibody is not used after reconstitution, the plate can be sealed and store at 2 - 8°C for a month in the dark. Once reconstituted, re-drying is not recommended as this may result in a loss of signal.

I have added my own antibody solution to the lyophilized product, will the lyophilized antibody work?

Yes, as long as the fluorophores on these antibodies are compatible and proper compensation has been applied during acquisition and analysis.

If I don't have enough cells and use much less than 4 million/mL (~ 3 x 105 cells/well), will it still work?

This may work with lower numbers of total cells, but we recommend trying to keep higher concentrations of cells for faster analysis. Of course, how many cells are needed depends on the specific application. Successful staining has been done with 1 x 105 cells/well.

What are the guarantees regarding the lyophilized plate compared to the reconstituted plate?

Lyophilized product has a guaranteed shelf life of 6 months unopened. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month. Be sure to properly seal the plates to prevent evaporation and shield the antibodies from light.

What is the level of variability from one experiment to the other?

If the protocol is followed closely, the variability should be minimal. The variability should be similar to single vial antibody staining.

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07-18-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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