LEGENDScreen™ Human PE Kit

Pricing & Availability
Regulatory Status
Ave. Rating
Submit a Review
Product Citations
Cat # Size Price Quantity Check Availability Save
700011 1 kit 1320€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

BioLegend's LEGENDScreen™ Human Cell PE Kit contains 354 PE-conjugated monoclonal antibodies to cell surface markers as well as 10 mouse, rat, or hamster Ig isotype controls. All of the antibodies have been pre-titrated to optimal concentrations, arrayed on four 96-well plates, and lyophilized in a ready-to-use format. This kit can be used to screen cell lines and primary cells (whole blood, PBMC, tumor cells etc.). There are blank wells on the plates for users to add their choice of markers. The end user can also choose to screen cells of interest directly or co-stain with other markers labeled with different fluorochromes. Positive "hits" from the screening can be quickly identified based on the plate map, and the catalog number and clone information can be obtained using the tables in the product manual. Analysis with individual fluorochrome-conjugated antibodies should be performed to confirm the screening results.

The LEGENDScreen™ Human Cell Screening (PE) Kit provides a convenient, easy-to-use, and powerful tool for immunology, stem cell, and cancer research.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Lyophilized antibodies in 96-well plate
Storage & Handling
Store unopened kit components at 2 - 8°C. Do not use this kit beyond its expiration date. Once opened, reconstitute plates with distilled, deionized water. The reconstituted plates can be used immediately or sealed for storage at 2 - 8°C in the dark for up to one month. Keep the buffer(s) at 2 - 8°C and use within one month after opening.

Cell Screening

Application Notes

Materials Provided in Kit:
4 Human Cell Screening Plates containing:
· 354 PE-conjugated Monoclonal Antibodies
· 10 Mouse, Rat, or Hamster Ig Isotype Controls
Cell Staining Buffer (Cat. No. 420201)
Fixation Buffer (Cat. No. 420801)
Plate Sealers (8 sheets)

Additional Product Notes

 Download the Excel Version of the Specificity/Clone List

Antigen Details

Biology Area
Molecular Family
CD Molecules
Gene ID

Related FAQs

If I don't have enough cells and use much less than 4 million/mL (~ 3 x 105 cells/well), will it still work?

This may work with lower numbers of total cells, but we recommend trying to keep higher concentrations of cells for faster analysis. Of course, how many cells are needed depends on the specific application. Successful staining has been done with 1 x 105 cells/well.

What are the guarantees regarding the lyophilized plate compared to the reconstituted plate?

Lyophilized product has a guaranteed shelf life of 6 months unopened. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month. Be sure to properly seal the plates to prevent evaporation and shield the antibodies from light.

How should the kit be stored?

The kit should be stored at 2 - 8°C upon receipt. Once opened, the plates must be reconstituted immediately. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month.

I have added my own antibody solution to the lyophilized product, will the lyophilized antibody work?

Yes, as long as the fluorophores on these antibodies are compatible and proper compensation has been applied during acquisition and analysis.

I am not going to use all the reconstituted antibody solution. Can I keep the left over for later or re-dry the solution in dark?

The antibody is in a one test per well format. There will not be any antibody left if the full test is used. Customers may decide to use less than the recommended volume per test, but this is not recommended and the performance is not guaranteed. Customers may also selectively transfer certain antibodies from the original plate to a new plate and use after reconstitution. If any antibody is not used after reconstitution, the plate can be sealed and store at 2 - 8°C for a month in the dark. Once reconstituted, re-drying is not recommended as this may result in a loss of signal.

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Can I use my existing institution discount for this new product?


Can I use half or less of the plate and keep the rest for later?

Yes. Customers can use half of the plate or whatever specificities they are interested in. However, the whole plate should be reconstituted. The half plate of antibodies must be transferred to another empty plate for the staining. The remaining half must be sealed and stored at 2 - 8°C in the dark and used within a month.

Are these plates made under sterile conditions?

The plates are not sterile. You would handle them as you would handle most typical flow cytometry staining protocols.

How do I request a custom LEGENDScreen™ product with only my specificities of interest?

For more info, visit: biolegend.com/custom_solutions.

What is the level of variability from one experiment to the other?

If the protocol is followed closely, the variability should be minimal. The variability should be similar to single vial antibody staining.

Go To Top Version: 1    Revision Date: 09-27-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account