APC/Cyanine7 anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
GK15_APCCyanine7_CD4_Antibody_020119
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD4 (clone GK1.5) APC/Cyanine7 (left) or Rat IgG2b, ? APC/Cyanine7 isotype control (right).
  • GK15_APCCyanine7_CD4_Antibody_020119
    C57BL/6 mouse splenocytes were stained with CD3 FITC and CD4 (clone GK1.5) APC/Cyanine7 (left) or Rat IgG2b, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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100413 25 µg 70€
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100414 100 µg 172€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  2. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  3. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  4. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  5. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  6. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  7. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  8. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  9. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  10. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  11. Shao B, et al. 2018. Mol Med Rep. 18:920. PubMed
  12. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  13. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  14. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  15. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  16. Dong L, et al. 2021. Cancer Cell. . PubMed
  17. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  18. Wang Q, et al. 2020. Wound Repair Regen. 28:105. PubMed
  19. Saito S, et al. 2020. Nutrients. 12:. PubMed
  20. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  21. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  22. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  23. Treger RS, et al. 2020. Immunity. 50(2):334-347.e9.. PubMed
  24. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  25. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  26. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  27. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  28. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  29. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  30. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  31. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  32. Yu X, et al. 2022. J Extracell Vesicles. 11:e12185. PubMed
  33. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  34. Feng H, et al. 2020. J Dermatol. 47:147. PubMed
  35. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  36. Collinson-Pautz M, et al. 2016. PLoS One. 11:e0164547. PubMed
  37. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  38. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  39. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  40. Levescot A, et al. 2021. J Clin Invest. 131:. PubMed
  41. Levi J, et al. 2020. J Nucl Med. . PubMed
  42. Poczobutt J, et al. 2016. J Immunol. 196: 891 - 901. PubMed
  43. Holvoet B, et al. 2015. Stem Cell Reports. 5: 1183-1195. PubMed
  44. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  45. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  46. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  47. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  48. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  49. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
  50. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  51. Mara AB, et al. 2022. NPJ Vaccines. 7:86. PubMed
  52. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  53. Zhang L, et al. 2021. Clin Transl Med. 11:e464. PubMed
  54. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  55. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  56. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  57. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  58. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  59. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  60. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  61. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  62. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  63. Brown SM et al. 2017. Endocrinology. 158(10):3592-3604 . PubMed
  64. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  65. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  66. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  67. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  68. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  69. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  70. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  71. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  72. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  73. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  74. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  75. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  76. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  77. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  78. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  79. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  80. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  81. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  82. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  83. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  84. Wang X, et al. 2021. Cell. 184:5357. PubMed
  85. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  86. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  87. McNamee E, et al. 2013. Gut. 62:53. PubMed
  88. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  89. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  90. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  91. Le QV, et al. 2022. Bioact Mater. 15:160. PubMed
  92. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  93. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  94. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  95. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  96. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  97. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  98. Chen B, et al. 2021. Cell. 184:6262. PubMed
  99. Wang X, et al. 2022. Elife. 11:. PubMed
  100. Ma C, et al. 2021. J Exp Med. :218. PubMed
  101. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  102. Sheng J, et al. 2021. eLife. 10:00. PubMed
  103. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  104. Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
  105. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  106. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  107. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  108. Ma C, et al. 2020. Sci Adv. 6:eaaz6717. PubMed
  109. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  110. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  111. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  112. Liang Z, et al. 2022. iScience. 25:105233. PubMed
  113. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
  114. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  115. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  116. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
RRID
AB_312698 (BioLegend Cat. No. 100413)
AB_312699 (BioLegend Cat. No. 100414)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC, IHC-F, ICC
FITC anti-mouse CD4 GK1.5 FC, IHC-F, ICC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC, IHC-F, ICC, IP, Costim, Block, Depletion
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC, IHC-F, ICC
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC, IHC-F, ICC
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC, ICC, IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC, Block, Costim, Depletion, IHC, IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F, FC, ICC, SB
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC, IHC-F, ICC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Go To Top Version: 2    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD4

  • Biotin anti-mouse CD4

  • FITC anti-mouse CD4

  • PE anti-mouse CD4

  • PE/Cyanine5 anti-mouse CD4

  • Purified anti-mouse CD4

  • PE/Cyanine7 anti-mouse CD4

  • APC/Cyanine7 anti-mouse CD4

  • Alexa Fluor® 647 anti-mouse CD4

  • Alexa Fluor® 488 anti-mouse CD4

  • Pacific Blue™ anti-mouse CD4

  • Alexa Fluor® 700 anti-mouse CD4

  • PerCP anti-mouse CD4

  • PerCP/Cyanine5.5 anti-mouse CD4

  • Brilliant Violet 421™ anti-mouse CD4

  • Ultra-LEAF™ Purified anti-mouse CD4

  • Alexa Fluor® 594 anti-mouse CD4

  • Brilliant Violet 711™ anti-mouse CD4

  • Brilliant Violet 510™ anti-mouse CD4

  • Brilliant Violet 605™ anti-mouse CD4

  • Brilliant Violet 785™ anti-mouse CD4

  • PE/Dazzle™ 594 anti-mouse CD4

  • APC/Fire™ 750 anti-mouse CD4

  • GoInVivo™ Purified anti-mouse CD4

  • Brilliant Violet 750™ anti-mouse CD4

  • Brilliant Violet 650™ anti-mouse CD4

  • Spark Blue™ 550 anti-mouse CD4

  • Spark NIR™ 685 anti-mouse CD4

  • KIRAVIA Blue 520™ anti-mouse CD4

  • PE/Fire™ 640 anti-mouse CD4

  • APC/Fire™ 810 anti-mouse CD4

  • PE/Fire™ 700 anti-mouse CD4

  • Spark Violet™ 538 anti-mouse CD4

  • Spark YG™ 593 anti-mouse CD4

  • Spark Blue™ 574 anti-mouse CD4 Antibody

  • Spark UV™ 387 anti-mouse CD4

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