LEGEND MAX™ Rat IL-6 ELISA Kit

Pricing & Availability
Other Names
IL-6 Pre-coated ELISA Kit
Ave. Rating
1 reviews
Product Citations
publications
Rat_IL-6_122910
  • Rat_IL-6_122910
Cat # Size Price Save
437107 1 Pre-coated Plate ¥70,000
Description

Interleukin 6 (IL-6) is a prototypic member of the IL-6 superfamily of cytokines. It is a pleiotropic cytokine that plays important roles in immune response, inflammation, hematopoiesis, bone metabolism, nerve cell functions, and cancer progression.

IL-6 is secreted by a variety of lymphoid and non-lymphoid cells including T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, keratinocytes, etc. Mature rat IL-6 is a 187 aa residue protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6. The production of IL-6 can be induced by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines, and viruses.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a subunit (gp130) that acts as the universal signal-transducing element for all IL-6 family cytokines. Binding of IL-6 to IL-6 R recruits gp130 to form a trimeric complex that dimerizes to form a hexameric complex, which transduces IL-6 signal. Binding of IL-6 to soluble IL-6R can also induce trans-signaling, which elicit response from gp130-expressing cells that lack of cell surface IL-6R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous.

IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. IL-6 is a key factor that reciprocally regulates Th17 and FOXP3(+) Treg differentiation by inhibition of TGF-β induced FOXP3 and induction of RORγ, a Th17 lineage-specific transcription factor.

Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations, and malignancies.

BioLegend's LEGEND MAX™ Rat IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which a rat IL-6-specific polyclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend's LEGEND MAX™ Rat IL-6 ELISA Kit is specifically designed for the accurate quantitation of rat IL-6 from cell culture supernatant, serum, plasma, or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Rat IL-6 Pre-coated 96-well Strip Microplate
  • Rat IL-6 Dectection Antibody
  • Rat IL-6 Standard
  • Matrix C (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Rat
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Buckley MM, et al. 2014. J Physiol. 592:5235. PubMed
Product Citations
  1. Buckley M, et al. 2014. J Physiol. 592:5235. PubMed
  2. Sverrisson K, et al. 2015. Am J Physiol Renal Physiol. 309: 800 - 806. PubMed
  3. Wu W, et al. 2017. Acta Biomaterialia. 10.1016/j.actbio.2017.05.024. PubMed
Publication Library
Sensitivity
5.3 pg/mL
Standard Range
18.8-1,200 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, keratinocytes and muscle cells
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Gene ID
24498 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 3    Revision Date: 01/16/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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