Pricing & Availability
Regulatory Status
Other Names
Rat TNF-α Pre-coated ELISA Kit
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Product Citations
  • Rat_TNF-a_050611
Cat # Size Price Quantity Check Availability Save
438207 1 Pre-coated Plate 276€
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TNF-a (Tumor Necrosis Factor a), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells and tumor cells. TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-a binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-a plays a critical role in many physiological and pathological conditions, including neurodegenerative diseases and cancer.

This kit is specifically designed to meet the increasing usage of rat models in biomedical studies due to larger sampling sizes, and the fact that rats have higher tolerance in chemotherapy, etc.

BioLegend’s Rat TNF-a LEGEND MAX™ ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96 well microtiter strip plates that are pre-coated with a capture antibody.

This ELISA kit is specifically designed for the accurate quantitation of Rat TNF-a from cell culture supernatant, serum, plasma or other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Rat TNF-α Pre-coated 96-well Strip Microplate
  • Rat TNF-α Dectection Antibody
  • Rat TNF-α Standard
  • Matrix A
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Application References
  1. Chi H, et al. 2013. Life Sci. 93:986. PubMed
Product Citations
  1. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  2. Roberto Rodrigues Bicalho P, et al. 2020. Surg Res Pract. 2020:5738236. PubMed
  3. Zhang C, et al. 2022. Front Pharmacol. 12:790072. PubMed
  4. Jantaruk P, et al. 2017. PLoS One.. 10.1371/journal.pone.0183852. PubMed
  5. Yadav A, et al. 2021. J Neuroinflammation. 18:238. PubMed
  6. Chia H, et al. 2013. Life Sci . 93:986. PubMed
  7. Lee SY, et al. 2022. Int J Mol Sci. 23:. PubMed
  8. Urkan M, et al. 2021. Ulus Travma Acil Cerrahi Derg. 27:284. PubMed
  9. Kumari A, Dash D, Singh R 2017. Inflammopharmacology. 10.1007/s10787-017-0334-3. PubMed
  10. Huang KW, et al. 2020. Mol Ther Nucleic Acids. 1.042361111. PubMed
  11. Chen CY, et al. 2021. Antioxidants (Basel). 10:. PubMed
  12. Song J, et al. 2020. Mol Med Rep. 1.400694444. PubMed
  13. Yamanaka D, et al. 2017. Journal of Anesthesia. 10.1007/s00540-017-2374-7. PubMed
  14. Peng KT, et al. 2019. Clin Sci (Lond). 133:2171. PubMed
  15. Huang NC, et al. 2021. Int J Mol Sci. 22:. PubMed
4.2 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
301289 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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