Purified anti-mouse / rat TNF-α Antibody

Pricing & Availability
Clone
TN3-19.12 (See other available formats)
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
Isotype
Armenian Hamster IgG
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
506101 50 µg 68€
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506102 500 µg 184€
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-α (TNF-α) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Cross-Reactivity: Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate or serial dilutions of rat TNF-α recombinant protein ranging from 2500 to 20 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1: The Ultra-LEAF™ Purified TN3-19.12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated Poly5160 antibody (Cat. No. 516003) as the detecting antibody for detecting mouse/rat TNF-α. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Clone TN3-19.12 has been shown to neutralize murine TNF-α and cross reacts wtih mouse TFN-β1. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Aside free, 0.2 µm filtered) is recommended for neutralization (Cat. Nos. 506109 - 506114).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan, K., et al. 1989. J. Immunol. 142:3884. (Neut)
  2. Merrick, B.A., et al. 1992. Appl. Theor. Electrophor 2:177.
  3. Takahashi, S., et al. 1995. Brain Res 690:241.
  4. Rabinovich, R., et al. 1993. J. Pharmacol. Exp. Ther. 267:1550.
  5. Molloy, R., et al. 1993. J. Immunol. 151:2142.
  6. Finkelman, F., et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  7. Simkins, HM., et al. 2011. J. Leukoc Biol. 89:753. PubMed.
  8. Faas MM, et al. 2003. J. Reprod. Immunol. 1:39-52.
Product Citations
  1. Leschner S, et al. 2009. PLoS One. 4:e6692. PubMed
  2. Simkins H, et al. 2011. J Leukoc Biol. 89:753. PubMed
Publication Library
RRID
AB_315415 (BioLegend Cat. No. 506101)
AB_315416 (BioLegend Cat. No. 506102)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 18-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID
21926 View all products for this Gene ID 24835 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

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