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Purified anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
A17188B (See other available formats)
Regulatory Status
RUO
Other Names
PD1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17188B_PURE_CD279_Antibody_102119
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with purified anti-human CD279 (clone A17188B, left) or mouse IgG2b, κ isotype control (right) followed by anti-mouse IgG PE. Cells were finally co-stained with CD3 APC.
  • A17188B_PURE_CD279_Antibody_102119
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with purified anti-human CD279 (clone A17188B, left) or mouse IgG2b, κ isotype control (right) followed by anti-mouse IgG PE. Cells were finally co-stained with CD3 APC.
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621602 100 µg 72€
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Description

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD279 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.

Product Citations
  1. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
RRID
AB_2820103 (BioLegend Cat. No. 621602)

Antigen Details

Structure
55 kD type I transmembrane protein
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling and co-stimulation (co-inhibition)
Interaction
SHP-1 and SHP-2
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887
  2. Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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