PE anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
bc96
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
  • bc96
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
302605 25 tests 32€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
302606 100 tests 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed
  2. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  3. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  4. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
  5. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  6. Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed
  7. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  8. Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
  9. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  10. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  11. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  12. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  13. Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed
  14. Wei H, et al. 2021. Malar J. 20:89. PubMed
  15. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  16. Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed
  17. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  18. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  19. Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
  20. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  21. Lopatina T, et al. 2018. Sci Rep. 8:17458. PubMed
  22. Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed
  23. Pothlichet J, et al. 2022. Front Immunol. 13:824746. PubMed
  24. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  25. Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed
  26. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  27. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  28. James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed
  29. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  30. Zhang W, et al. 2012. J Biol Chem. 287:34157. PubMed
  31. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  32. Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed
  33. Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
  34. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  35. Robertson N, et al. 2020. J Biol Chem. . PubMed
  36. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  37. Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed
  38. Erdem A, et al. 2022. Nat Commun. 13:1105. PubMed
  39. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  40. Carnevale J, et al. 2022. Nature. 609:174. PubMed
  41. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  42. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  43. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  44. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  45. Mengrelis K, et al. 2021. Pediatr Transplant. 25:e13930. PubMed
  46. Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
  47. Reichwald K, et al. 2014. PLoS One. 9:105627. PubMed
  48. Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed
  49. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  50. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  51. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  52. Keefe RC, et al. 2021. Sci Rep. 11:14933. PubMed
  53. Tian M, et al. 2021. Elife. 10:. PubMed
  54. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  55. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  56. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  57. Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed
  58. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  59. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  60. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  61. Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed
  62. Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
  63. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  64. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  65. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  66. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  67. Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed
  68. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  69. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  70. Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed
  71. Cerny D, et al. 2016. J Allergy Clin Immunol. 138:1709-1712. PubMed
  72. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  73. Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed
  74. Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed
  75. Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed
  76. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
  77. Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed
  78. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
RRID
AB_314275 (BioLegend Cat. No. 302605)
AB_314276 (BioLegend Cat. No. 302606)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account