- BC96 (See other available formats)
- Regulatory Status
- V T-072
- Other Names
- Low affinity IL-2R, IL-2R α chain, Tac, p55
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.Product Details
- Verified Reactivity
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications include: immunocytochemistry3.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_493754 (BioLegend Cat. No. 302621)
AB_493755 (BioLegend Cat. No. 302622)
- Type I transmembrane glycoprotein, 55 kD
Activated T cells and B cells, monocytes/macrophages, Treg
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- View information about CD25 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD25 Reagents Request Custom Conjugation
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 647 anti-human CD25
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Brilliant Violet 605™ anti-human CD25
Brilliant Violet 650™ anti-human CD25
Brilliant Violet 711™ anti-human CD25
Brilliant Violet 785™ anti-human CD25
Brilliant Violet 510™ anti-human CD25
APC/Fire™ 750 anti-human CD25
TotalSeq™-A0085 anti-human CD25
PE/Dazzle™ 594 anti-human CD25
TotalSeq™-B0085 anti-human CD25
TotalSeq™-C0085 anti-human CD25
TotalSeq™-D0085 anti-human CD25