- GL-1 (See other available formats)
- Regulatory Status
- Other Names
- B7-2, B70, Ly-58
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- LPS-activated CBA/Ca mouse splenic B cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).
(PubMed link indicates BioLegend citation)
- Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
- Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
- Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
- Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
- Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
- Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
- Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Turnquist HR, et al. 2007. J. Immunol. 178:7018.
- Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
- de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
- Product Citations
AB_493465 (BioLegend Cat. No. 105019)
AB_493464 (BioLegend Cat. No. 105020)
- Ig superfamily, 80 kD
B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
- T cell costimulation, Ig class-switching, NK cell cytotoxicity
- CD28, CD152 (CTLA-4)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
- Gene ID
- 12524 View all products for this Gene ID
- View information about CD86 on UniProt.org
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Brilliant Violet 650™ anti-mouse CD86
Biotin anti-mouse CD86
FITC anti-mouse CD86
PE anti-mouse CD86
Purified anti-mouse CD86
Brilliant Violet 605™ anti-mouse CD86
APC anti-mouse CD86
PE/Cyanine7 anti-mouse CD86
Alexa Fluor® 488 anti-mouse CD86
Alexa Fluor® 647 anti-mouse CD86
Pacific Blue™ anti-mouse CD86
PE/Cyanine5 anti-mouse CD86
Alexa Fluor® 700 anti-mouse CD86
PerCP/Cyanine5.5 anti-mouse CD86
PerCP anti-mouse CD86
APC/Cyanine7 anti-mouse CD86
Brilliant Violet 421™ anti-mouse CD86
Brilliant Violet 510™ anti-mouse CD86
PE/Dazzle™ 594 anti-mouse CD86
Brilliant Violet 785™ anti-mouse CD86
APC/Fire™ 750 anti-mouse CD86
TotalSeq™-A0200 anti-mouse CD86
TotalSeq™-B0200 anti-mouse CD86
Ultra-LEAF™ Purified anti-mouse CD86
TotalSeq™-C0200 anti-mouse CD86