PE anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
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Product Citations
publications
PC61_PE_080607
Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 PE
  • PC61_PE_080607
    Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
102007 50 µg 55 CHF
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102008 200 µg 190 CHF
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  2. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  3. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  4. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  5. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  6. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  7. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  8. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  9. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  10. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  11. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  12. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  13. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  14. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  15. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  16. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  17. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  18. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  19. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  20. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  21. Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed
  22. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  23. O'Malley G, et al. 2018. Cancer Immunol Res. 1.240277778. PubMed
  24. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  25. Tong D, et al. 2020. Protein Cell. 0.533333333. PubMed
  26. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  27. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  28. Elong Ngono A, et al. 2020. Cell Reports. 1.330555556. PubMed
  29. Imanishi T, et al. 2020. Cell Reports. 32(3):107911. PubMed
  30. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  31. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  32. Merck E, et al. 2011. J Immunol. 187:110. PubMed
  33. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  34. White C, et al. 2015. J Immunol. 194:697. PubMed
  35. Singh K, et al. 2015. Sci Rep. 14:7767. PubMed
  36. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  37. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  38. Ibrahim M, et al. 2016. J Evid Based Complementary Altern Med. 21: 171 - 176. PubMed
  39. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  40. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  41. Flaherty S, Reynolds J 2015. J Vis Exp. 98: 52739. PubMed
  42. Holvoet B, et al. 2015. Stem Cell Reports. 5: 1183-1195. PubMed
  43. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  44. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  45. Orlanski S, et al. 2016. Proc Natl Acad Sci U S A. 113: 5018-5023. PubMed
  46. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  47. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  48. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  49. Takenori Inomata, Jing Hua, Antonio Di Zazzo 2016. Sci Rep. 6:39924. PubMed
  50. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
RRID
AB_312856 (BioLegend Cat. No. 102007)
AB_312857 (BioLegend Cat. No. 102008)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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