PE anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B4-1
PHA stimulated (3 days) peripheral blood mononuclear cells stained with 4B4-1 PE
  • 4B4-1
    PHA stimulated (3 days) peripheral blood mononuclear cells stained with 4B4-1 PE
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309803 25 tests 118 CHF
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309804 100 tests 252 CHF
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Liu T, et al. 2021. Gut. 70:1965. PubMed
  2. Shitaoka K, et al. 2018. Cancer Immunol Res. 6:378. PubMed
  3. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  4. Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed
  5. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  6. Goncharov MM, et al. 2022. Elife. 11:. PubMed
  7. Shin JJ, et al. 2022. J Clin Immunol. :. PubMed
  8. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  9. Pavlicek D, et al. 2017. Immun Ageing. 14:22. PubMed
  10. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
  11. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  12. Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed
  13. Elias G, et al. 2022. Elife. 11:. PubMed
  14. Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed
  15. Correnti CE, et al. 2020. Nat Struct Mol Biol. 27:342. PubMed
  16. Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed
  17. Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed
  18. Senger K, et al. 2022. Curr Protoc. 2:e538. PubMed
  19. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_314782 (BioLegend Cat. No. 309803)
AB_314783 (BioLegend Cat. No. 309804)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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