LEGEND MAX™ Rat IFN-γ ELISA Kit

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Other Names
Rat IFN-γ Pre-coated ELISA Kit
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Rat-IFNg_LM_092111
  • Rat-IFNg_LM_092111
Cat # Size Price Quantity Save
439007 1 Pre-coated Plate 295 CHF
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Description

Interferon-γ (IFN-γ) is the sole member of the type II interferon category. It is a potent multifunctional cytokine involved in both the innate and adaptive (especially cell-mediated) arms of immunity. It is secreted largely by activated lymphocytes, in particular, CD4+ Th1 cells, CD8+ cytotoxic cells, and NK cells.

Human IFN-γ is expressed as a pre-cursor protein with a signaling peptide which is cleaved to produce the mature protein of ~17 kD. Its active form is a homodimer, linked by anti-parallel non-covalent interactions between the six α-helices of each monomer. The receptor for IFN-γ is also a homodimer, with α & β subunits (IFNGR1 and IFNGR2, respectively). IFN-γ binds to IFNGR1 very specifically, and the subsequent interaction of IFNGR1 with IFNGR2 activates the Jak/Stat pathway for signal transduction and, ultimately, transcription of effector genes. Rat IFN-γ has 87% amino acid identity with mouse IFN-γ and 39% identity with the human protein. As such, rat IFN-γ can activate mouse cells but not human cells.

Originally characterized based on anti-viral properties, IFN-γ also exerts anti-microbial, anti-tumor, immunomodulatory, and pro-inflammatory activities. It is unique among the interferons for its ability to coordinate the transition from innate to adaptive immunity. Among its more significant roles, IFN-γ stimulates macrophage effector functions. It is also the major player in inducing Th1 cell differentiation and provides a positive feedback loop in response to IL-12 stimulation in defining Th1 cells.


BioLegend's LEGEND MAX™ Rat IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a rat IFN-γ-specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend's LEGEND MAX™ Rat IFN-γ ELISA Kit is specifically designed for the accurate quantitation of rat IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Rat IFN-γ Pre-coated 96-well Strip Microplate
  • Rat IFN-γ Dectection Antibody
  • Rat IFN-γ Standard
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Rat
Application

ELISA

Sensitivity
3.2 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
25712 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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