Brilliant Violet 650™ anti-human CD4 Antibody, CD4, SK3

Pricing & Availability

Clone: SK3 (See other available formats)
Regulatory Status: RUO
Other Names: T4, Leu3a
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK3_BV650_CD4_Antibody_FC_021523 — Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD4 (clone SK3) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ (right).

Compare all formats See Brilliant Violet 650™ spectral data

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344691

25 tests

185 CHF

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344692

100 tests

370 CHF

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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application References (PubMed link indicates BioLegend citation)

Evans RL, et al. 1981. Immunol. 78:544
Arno A et al. 1999. J. Infect. Dis. 180:56
Muech M, et al. 1997. Blood 89:1364
Wang L, et al. 2012. Cytometry A. 81:567. PubMed

RRID

AB_2936743 (BioLegend Cat. No. 344691)
AB_2936743 (BioLegend Cat. No. 344692)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
PerCP anti-human CD4	SK3	FC
Purified anti-human CD4	SK3	FC,IP
FITC anti-human CD4	SK3	FC
PE anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Biotin anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
APC/Cyanine7 anti-human CD4	SK3	FC
Alexa Fluor® 488 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Alexa Fluor® 700 anti-human CD4	SK3	FC
Purified anti-human CD4 (Maxpar® Ready)	SK3	FC,CyTOF®
PE anti-human CD4	SK3	FC
Brilliant Violet 421™ anti-human CD4	SK3	FC
Brilliant Violet 510™ anti-human CD4	SK3	FC
Alexa Fluor® 647 anti-human CD4	SK3	FC
FITC anti-human CD4	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
PE/Dazzle™ 594 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Brilliant Violet 785™ anti-human CD4	SK3	FC
Brilliant Violet 750™ anti-human CD4	SK3	FC
Brilliant Violet 605™ anti-human CD4	SK3	FC
Brilliant Violet 711™ anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
TotalSeq™-A0045 anti-human CD4	SK3	PG
TotalSeq™-C0045 anti-human CD4	SK3	PG
PE/Cyanine5 anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Spark Blue™ 550 anti-human CD4	SK3	FC
Spark NIR™ 685 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
KIRAVIA Blue 520™ anti-human CD4	SK3	FC
APC/Fire™ 810 anti-human CD4	SK3	FC
PE/Fire™ 640 anti-human CD4	SK3	FC
PE/Fire™ 700 anti-human CD4	SK3	FC
PerCP anti-human CD4	SK3	FC
Spark Violet™ 538 anti-human CD4 Antibody	SK3	FC
Spark YG™ 581 anti-human CD4	SK3	FC
Alexa Fluor® 660 anti-human CD4	SK3	FC
Spark YG™ 593 anti-human CD4	SK3	FC
PE/Fire™ 810 anti-human CD4 Antibody	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Blue™ 574 anti-human CD4	SK3	FC
GMP APC anti-human CD4	SK3	FC
TotalSeq™-B0045 anti-human CD4 Antibody	SK3	PG
Spark Violet™ 423 anti-human CD4 Antibody	SK3	FC
GMP Pacific Blue™ anti-human CD4	SK3	FC
Spark UV™ 387 anti-human CD4	SK3	FC
GMP PE anti-human CD4	SK3	FC
GMP FITC anti-human CD4	SK3	FC
Spark Red™ 718 anti-human CD4	SK3	FC
GMP PerCP anti-human CD4	SK3	FC
GMP PE/Cyanine7 anti-human CD4	SK3	FC
Spark Violet™ 500 anti-human CD4	SK3	FC
GMP PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Brilliant Violet 650™ anti-human CD4	SK3	FC
PerCP/Fire™ 806 anti-human CD4	SK3	FC
Spark Blue™ 515 anti-human CD4	SK3	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02.15.2023

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.