Brilliant Violet 421™ anti-mouse IL-33Rα (IL1RL1, ST2) Antibody

Pricing & Availability
Clone
DIH9 (See other available formats)
Regulatory Status
RUO
Other Names
T1/ST2, ST2L, IL-1R1, T1, DER4, IL1R1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
DIH9_BV421_Mouse_IL-33Ra_FC_043015.jpg
Mouse Th2 clone D10.G4.1 was stained with anti-mouse IL-33Ra/ST2 (clone DIH9) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • DIH9_BV421_Mouse_IL-33Ra_FC_043015.jpg
    Mouse Th2 clone D10.G4.1 was stained with anti-mouse IL-33Ra/ST2 (clone DIH9) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
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145309 50 µg 235,00 CHF
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Description

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts.

Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-33Rα-hFcγ1 fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed
Product Citations
  1. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  2. McCarthy PC, et al. 2019. Immunol Cell Biol. 97:54. PubMed
  3. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  4. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
  5. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
RRID
AB_2565634 (BioLegend Cat. No. 145309)

Antigen Details

Structure
63 kD, IL-1 receptor family member, single pass type I membrane protein
Distribution

Mast cells, Th2 cells, and fibroblasts

Function
Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway.
Interaction
Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor
IL-33
Cell Type
Fibroblasts, Mast cells, Th2
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83.
2. Schmitt E, et al. 1990. Cytokine 6:407.
3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51.
4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.

Gene ID
17082 View all products for this Gene ID
UniProt
View information about IL-33Ra on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04.30.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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