Purified anti-mouse/human NGF Antibody

Pricing & Availability
JKmNGF-1 (See other available formats)
Regulatory Status
Other Names
Nerve growth factor, NGF, β-NGF
Mouse IgG1, κ
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Product Citations
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509701 50 µg $90.00
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509702 500 µg $295.00
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Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight complex. This complex contains two identical 118-amino acid β-chains, which are solely responsible for nerve growth stimulating activity of NGF. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the functional integrity of cholinergic neurons in the central nervous system (CNS).

Product Details
Technical data sheet

Product Details

Mouse, Human, 2.5S, 7S, and β-NGF forms.
Antibody Type
Host Species
recombinant human NGF (a.a. 46-62)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 6 - 10 µg/ml is recommended. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified JKmNGF-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JKhNGF-2 antibody (Cat. No. 509802) as the detecting antibody for detecting mouse NGF.

AB_315543 (BioLegend Cat. No. 509701)
AB_2282783 (BioLegend Cat. No. 509702)

Antigen Details

Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian)
Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources
Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils
Cell Targets
Neurons, B cells, mast cells, eosinophils
Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA)
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205.
3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267.
4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369.

Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β
Gene ID
18049 View all products for this Gene ID 4803 View all products for this Gene ID
View information about NGF on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 07/22/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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