Purified anti-human CD25 Antibody

Pricing & Availability
Clone
TÜ69 (See other available formats)
Regulatory Status
RUO
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-TU69_PURE_CD25_Recombinant_Antibody_1_updated_060821.png
Peripheral blood lymphocytes were stained with anti-human CD4 APC and purified anti-human CD25 (clone TÜ69) (left) or mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE.
  • a-TU69_PURE_CD25_Recombinant_Antibody_1_updated_060821.png
    Peripheral blood lymphocytes were stained with anti-human CD4 APC and purified anti-human CD25 (clone TÜ69) (left) or mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE.
  • b-TU69_PURE_CD25_Antibody_2_060821.png
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone TÜ69) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
332502 100 µg $100.00
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Alloreactive activated T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Valent P, et al. 2014. Leuk Res Rep. 3:8-13. (IHC-F, IHC-P)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell. 73:5.
  2. Waldmann T. 1991. J Biol Chem. 266:2681.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/08/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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