PE anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_PE_040111
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PE (filled histogram), or rat IgG2c, κ PE isotype control (open histogram). Gated on myeloid cell population.
  • HK1dot4_PE_040111
    C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PE (filled histogram), or rat IgG2c, κ PE isotype control (open histogram). Gated on myeloid cell population.
See PE spectral data
Cat # Size Price Quantity Avail. Save
128007 50 µg $110.00
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128008 200 µg $275.00
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  2. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  3. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  4. Zhang YS, et al. 2018. Cancer Biol Ther. 19:735. PubMed
  5. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  6. Wang F, et al. 2018. Carcinogenesis. 39:889. PubMed
  7. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  8. Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed
  9. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  10. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  11. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  12. Kitamura K, et al. 2019. Int J Cardiol Heart Vasc. 23:100344. PubMed
  13. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
  14. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  15. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  16. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  17. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  18. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  19. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  20. Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
  21. Zuber J, et al. 2009. Genes Dev. 1.567361111. PubMed
  22. Younos I, et al. 2011. Int Immunopharmacol. 11:816. PubMed
  23. Petersen B, et al. 2014. J Leukoc Biol. 95:809. PubMed
  24. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  25. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  26. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  27. Koronyo Y, et al. 2015. Brain. 138: 2399-2422. PubMed
  28. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  29. Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed
  30. Kar S, Colino J, Snapper C 2016. J Immunol. 196: 4204 - 4213. PubMed
  31. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  32. Gousopoulos E, et al. 2016. Am J Pathol. 186:2193-2203. PubMed
  33. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  34. Francis M, et al. 2016. Toxicol Sci. 10.1093/toxsci/kfw226. PubMed
  35. Gerwing M, et al. 2020. Mol Imaging Biol. 1.959027778. PubMed
  36. Burns JC, et al. 2020. eLife. 9:00. PubMed
  37. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  38. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  39. Yurdagul A Jr, et al. 2020. Cell Metabolism. 31(3):518-533.e10. PubMed
  40. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  41. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  42. Wang Y, et al. 2020. Immunity. 53(6):1168-1181.e7. PubMed
  43. Liu Z, et al. 2021. Immunity. 54(2):247-258.e7. PubMed
  44. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
RRID
AB_1186133 (BioLegend Cat. No. 128007)
AB_1186132 (BioLegend Cat. No. 128008)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/09/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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