PerCP anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1.4_PerCP_040511
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PerCP. Data shown was gated on myeloid cell population.
  • HK1.4_PerCP_040511
    C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PerCP. Data shown was gated on myeloid cell population.
Compare all formats See PerCP spectral data
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128027 25 µg $136
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128028 100 µg $329
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Chinta KC et al. 2018. Cell reports. 25(7):1938-1952 . PubMed
  2. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  3. Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed
  4. Tsiganov E, et al. 2014. J Immunol. 192:4718. PubMed
  5. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  6. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  7. Zheng J, et al. 2020. bioRxiv. . PubMed
  8. Wheeler MA, et al. 2019. Cell. 176:581. PubMed
  9. Zheng J, et al. 2020. Nature. . PubMed
  10. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  11. Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
  12. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  13. Molgora M, et al. 2020. Cell. 182:886. PubMed
  14. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  15. Wheeler MA, et al. 2020. Nature. 578:593. PubMed
  16. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  17. Goddery EN, et al. 2021. Front Immunol. 12:726421. PubMed
RRID
AB_10900235 (BioLegend Cat. No. 128027)
AB_10897805 (BioLegend Cat. No. 128028)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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