PE anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_PE_040111
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PE (filled histogram), or rat IgG2c, κ PE isotype control (open histogram). Gated on myeloid cell population.
  • HK1dot4_PE_040111
    C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PE (filled histogram), or rat IgG2c, κ PE isotype control (open histogram). Gated on myeloid cell population.
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128007 50 µg $121
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128008 200 µg $303
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Younos I, et al. 2011. Int Immunopharmacol. 11:816. PubMed
  2. Petersen B, et al. 2014. J Leukoc Biol. 95:809. PubMed
  3. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  4. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  5. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  6. Koronyo Y, et al. 2015. Brain. 138: 2399-2422. PubMed
  7. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  8. Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed
  9. Kar S, Colino J, Snapper C 2016. J Immunol. 196: 4204 - 4213. PubMed
  10. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  11. Gousopoulos E, et al. 2016. Am J Pathol. 186:2193-2203. PubMed
  12. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  13. Francis M, et al. 2016. Toxicol Sci. 10.1093/toxsci/kfw226. PubMed
  14. Gerwing M, et al. 2020. Mol Imaging Biol. 1.959027778. PubMed
  15. Burns JC, et al. 2020. eLife. 9:00. PubMed
  16. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  17. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  18. Yurdagul A Jr, et al. 2020. Cell Metabolism. 31(3):518-533.e10. PubMed
  19. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  20. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  21. Wang Y, et al. 2020. Immunity. 53(6):1168-1181.e7. PubMed
  22. Liu Z, et al. 2021. Immunity. 54(2):247-258.e7. PubMed
  23. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  24. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  25. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  26. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  27. Zhang YS, et al. 2018. Cancer Biol Ther. 19:735. PubMed
  28. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  29. Wang F, et al. 2018. Carcinogenesis. 39:889. PubMed
  30. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  31. Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed
  32. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  33. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  34. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  35. Kitamura K, et al. 2019. Int J Cardiol Heart Vasc. 23:100344. PubMed
  36. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
  37. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  38. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  39. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  40. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  41. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  42. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  43. Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
  44. Zuber J, et al. 2009. Genes Dev. 1.567361111. PubMed
RRID
AB_1186133 (BioLegend Cat. No. 128007)
AB_1186132 (BioLegend Cat. No. 128008)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/09/2013

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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