- B02P6E6 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-27 p28, IL-27-A, IL-27 subunit alpha
- Rat IgG2a, κ
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- Product Citations
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IL-27, a heterodimer protein, belongs to the IL-12 cytokine family. IL-27 is composed of the Epstein-Barr virus-induced gene 3 (EBI-3), a 34 kD glycoprotein that is related to the p40 subunit of IL-12; and p28, a 28 kD glycoprotein that is related to the p35 subunit of IL-12 and p19 subunit of IL-23.
IL-27 triggers expansion of antigen-specific naïve CD4+ T cells, promotes polarization of Th1 cells, and inhibits the development of Th2 and Th17 cells. It is known that IL-27 is an early product of activated antigen-presenting cells (APCs). IL-27 plays an important role in regulating the activity of B and T lymphocytes.
- Antibody Type
- Host Species
- Recombinant mouse IL-27
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.125 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
- Application Notes
ELISA Detection: To measure mouse IL-27, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified MM27-7B1 antibody (Cat. No. 516902) as the capture antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.
Note: For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended.
AB_2563423 (BioLegend Cat. No. 525903)
AB_2563424 (BioLegend Cat. No. 525904)
- A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
- Produced by antigen-presenting cells, including macrophages and dendritic cells.
- Drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization and IFN-Î³ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
- T cells and NK cells.
- IL-27R (TCCR) (WSX-1).
- Cell Type
- Antigen-presenting cells, Macrophages, Dendritic cells, B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Antigen References
1. Salcedo R, et al. 2004. J. Immunol. 173:7170.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Pflanz S, et al. 2002. Immunity 16:779.
4. Diveu C, et al. 2009. J. Immunol. 182:5748.
5. Morishima N, et al. 2005. J. Immunol. 175:1686.
6. Liu J, et al. 2007. J. Exp. Med. 204:141.
- Gene ID
- 246779 View all products for this Gene ID
- View information about IL-27 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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