APC anti-mouse CD144 (VE-cadherin) Antibody

Pricing & Availability
Clone
BV13 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial-cadherin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
BV13_APC_101310
bEnd.3 cells surface stained with CD144 (clone BV13) APC (filled histogram) or rat IgG1 APC isotype control (open histogram).
  • BV13_APC_101310
    bEnd.3 cells surface stained with CD144 (clone BV13) APC (filled histogram) or rat IgG1 APC isotype control (open histogram).
See APC spectral data
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138011 25 µg $115.00
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138012 100 µg $270.00
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Description

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VE-cadherin-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.

Application References

(PubMed link indicates BioLegend citation)
  1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
  2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
  3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
  4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
  5. May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations
  1. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  2. Bibli SI, et al. 2020. Redox Biol. 28:101379. PubMed
  3. Gomez I, et al. 2020. Nat Commun. 11:214. PubMed
  4. Geis T, et al. 2014. Exp Cell Res. 331:46. PubMed
  5. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  6. Jurkin J, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31121-6. PubMed
  7. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  8. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
RRID
AB_10679039 (BioLegend Cat. No. 138011)
AB_10680420 (BioLegend Cat. No. 138012)

Antigen Details

Structure
A 120 kD member of type II Cadherin family
Distribution

Endothelial cells

Function
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor
CD144
Cell Type
Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.

Gene ID
12562 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01/12/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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