Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) Antibody

Pricing & Availability
Clone
BV13 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial-cadherin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
BV13_AF647_042710
Mouse endothelial cells b.End.3 stained with BV13 Alexa Fluor® 647
  • BV13_AF647_042710
    Mouse endothelial cells b.End.3 stained with BV13 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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138005 25 µg $176
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138006 100 µg $411
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Description

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VE-cadherin-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.

Application References

(PubMed link indicates BioLegend citation)
  1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
  2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
  3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
  4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
  5. May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations
  1. Himburg HA et al. 2018. Cell stem cell. 23(3):370-381 . PubMed
  2. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  3. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  4. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  5. Himburg HA, et al. 2019. J Clin Invest. 130:315. PubMed
  6. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  7. Rybtsov S, et al. 2011. J Exp Med. 208:1305. PubMed
  8. Kedem A, et al. 2017. J Ovarian Res. 10.1186/s13048-017-0354-z. PubMed
  9. Himburg H, et al. 2016. Nat Microbiol. 2:16232. PubMed
  10. Poulos M, et al. 2016. Nat Commun. 7:13829. PubMed
  11. Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed
  12. Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
  13. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  14. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  15. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  16. Zhang J, et al. 2021. Nature. 590:457. PubMed
  17. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  18. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  19. Gordon-Keylock S, et al. 2013. Blood. 122:2338. PubMed
  20. Gao X, et al. 2021. Nature. 589:591. PubMed
  21. Renner B, et al. 2013. J Am Soc Nephrol. 24:1849. PubMed
  22. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  23. Mizoguchi T, et al. 2014. Dev Cell. 29:340. PubMed
  24. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  25. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID
AB_10568319 (BioLegend Cat. No. 138005)
AB_10569114 (BioLegend Cat. No. 138006)

Antigen Details

Structure
A 120 kD member of type II Cadherin family
Distribution

Endothelial cells

Function
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor
CD144
Cell Type
Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.

Gene ID
12562 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 01/12/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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