Alexa Fluor® 647 anti-mouse CD107b (Mac-3) Antibody

Pricing & Availability
M3/84 (See other available formats)
Regulatory Status
Other Names
CD107b, LAMP-2
Rat IgG1, κ
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Product Citations
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 647
  • a_M3slash84_Alx647_083107
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 647
  • b_M384_A647_CD170b_Antibody_ICC_080719
    Thioglycolate-elicited C57BL/6 mouse peritoneal cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of anti-mouse CD107b (Mac-3) (clone M3/84) Alexa Fluor® 647 (blue) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI (red). The image was captured with 60X objective.
See Alexa Fluor® 647 spectral data
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108511 25 µg $85.00
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108512 100 µg $195.00
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Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.

Application References

(PubMed link indicates BioLegend citation)
  1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
  2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
  3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
  4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
  7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
  8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
  9. Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
  10. Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
  11. Nessler S, et al. 2007. Brain 130:2186. (IHC)
Product Citations
  1. Durrani–Kolarik S, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L339. PubMed
  2. Shang W, et al. 2019. J Diabetes Res. 2019:5641271. PubMed
  3. Tay S, et al. 2014. Proc Natl Acad Sci U S A. 111:2540. PubMed
AB_493311 (BioLegend Cat. No. 108511)
AB_493310 (BioLegend Cat. No. 108512)

Antigen Details

110 kD

Macrophages, some myeloid cell lines

Differentiation and activation
Cell Type
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.

Gene ID
16784 View all products for this Gene ID
View information about CD107b on

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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