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Alexa Fluor® 647 anti-mouse CD107b (Mac-3) Antibody

Pricing & Availability
Clone
M3/84 (See other available formats)
Regulatory Status
RUO
Other Names
CD107b, LAMP-2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
a_M3slash84_Alx647_083107
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 647
  • a_M3slash84_Alx647_083107
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 647
  • b_M384_A647_CD170b_Antibody_ICC_080719
    Thioglycolate-elicited C57BL/6 mouse peritoneal cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of anti-mouse CD107b (Mac-3) (clone M3/84) Alexa Fluor® 647 (blue) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI (red). The image was captured with 60X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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108511 25 µg $101
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108512 100 µg $201
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Description

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.

Application References

(PubMed link indicates BioLegend citation)
  1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
  2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
  3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
  4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
  7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
  8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
  9. Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
  10. Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
  11. Nessler S, et al. 2007. Brain 130:2186. (IHC)
Product Citations
  1. Tay S, et al. 2014. Proc Natl Acad Sci U S A. 111:2540. PubMed
  2. Shang W, et al. 2019. J Diabetes Res. 2019:5641271. PubMed
  3. Miyawaki D, et al. 2022. Heliyon. 8:e10644. PubMed
  4. Durrani–Kolarik S, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L339. PubMed
RRID
AB_493311 (BioLegend Cat. No. 108511)
AB_493310 (BioLegend Cat. No. 108512)

Antigen Details

Structure
110 kD
Distribution

Macrophages, some myeloid cell lines

Function
Differentiation and activation
Cell Type
Macrophages
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.

Gene ID
16784 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 08/07/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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