Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) Antibody (Previously Covance catalog# A488-435L)

Pricing & Availability
Clone
TUJ1 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# A488-435L
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1_TUJ1_AF488_Tubulin_B_3_Antibody_IHC_011615
CHO cell line expressing Tubulin β 3 stained with Alexa Fluor® 488 anti-Tubulin β 3.
  • 1_TUJ1_AF488_Tubulin_B_3_Antibody_IHC_011615
    CHO cell line expressing Tubulin β 3 stained with Alexa Fluor® 488 anti-Tubulin β 3.
  • 2_TUJ1_A488_Tubulinbeta3_Antibody_IHC-P_2_011718.jpg
    IHC staining of Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. The image was captured with a 40X objective.
  • 3_TUJ1_A488_Tubulinbeta3_Antibody_IHC-P_3_011718.jpg
    IHC staining of Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) antibody (Clone TUJ1) on formalin-fixed paraffin-embedded normal human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. The image was captured with a 40X objective.
  • 4_TUJ1_A488_Tubulinbeta3_Antibody_4_101420
    Human lung adenocarcinoma cell line A549 was treated with Fixation Buffer (Cat# 420801) and Permeabilization Wash Buffer (Cat# 421002), and then stained with TUBB3 (clone TUJ1) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
  • TUJ1_A488_Tubulinbeta3_Antibody_3D_IHC_05112021.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse kidney tissue section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-Tubulin β 3 (TUBB3) Antibody (clone TUJ1) Alexa Fluor® 488 at 5 µg/mL (green), and anti-mouse CD326 (Ep-CAM) Antibody (clone G8.8) Alexa Fluor® 594 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 488 spectral data
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801203 100 µL $300.00
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Alexa Fluor® Labeled Antibody (in PBS + 50% glycerol + 0.03% thimerosal).
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P, ICFC, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution of 1:500 is recommended. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, and Western blotting8.

This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
  2. Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
  3. Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
  4. Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
  5. Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
  6. Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
  7. Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
  8. Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
Product Citations
  1. Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed
  2. McCurdy EP et al. 2019. Cell Rep. 29(2):363-377 . PubMed
  3. Larhammar M et al. 2017. eLife. 6 pii: e20725. PubMed
  4. Geisler S, et al. 2019. JCI Insight. 4:e129920. PubMed
  5. Rocktäschel P, et al. 2019. Epilepsy Behav. 101:106581. PubMed
  6. Arsić A, et al. 2020. Sci Rep. 10:6441. PubMed
  7. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  8. Stachtea X, et al. 2015. PLoS One. 10: e0140279. PubMed
  9. Giacomini C, et al. 2016. Mol Biol Cell. 27: 35 - 47. PubMed
  10. Kim Y, et al. 2016. Sci Rep. 6: 23799. PubMed
  11. Lu R, et al. 2021. Int J Mol Sci. 22:00. PubMed
  12. Inlender T, et al. 2021. Scientific Reports. 11(1):12796. PubMed
  13. Žiak J, et al. 2020. EMBO Rep. e48512:21. PubMed
RRID
AB_2564757 (BioLegend Cat. No. 801203)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Structure
Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.

Function
Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction
Alpha tubulin, kinesin and dynein.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 05/11/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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