Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) Antibody

Pricing & Availability
Clone
TUJ1 (See other available formats)
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_TUJ1_A647_Tubulinbeta3_Antibody_040617
ICC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% Normal Goat Serum. The cells were then stained with 1 µg/mL of the primary antibody for three hours at room temperature. Nuclei were counterstained with Hoechst 33342. The image was captured with a 60X objective.
  • 1_TUJ1_A647_Tubulinbeta3_Antibody_040617
    ICC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% Normal Goat Serum. The cells were then stained with 1 µg/mL of the primary antibody for three hours at room temperature. Nuclei were counterstained with Hoechst 33342. The image was captured with a 60X objective.
  • 2_TUJ1_A647_Tubulinbeta3_Antibody_010818
    ICC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 1 µg/ml of the primary antibody for three hours at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
See Alexa Fluor® 647 spectral data See high resolution ICC data...
Cat # Size Price Quantity Avail. Save
801209 25 µg $120
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801210 100 µg $300
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, and Western blotting8.

This antibody is well characterized and highly reactive to neuron specific Class III β-tubulin (βIII). TUJ1 does not identify β-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
  2. Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
  3. Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
  4. Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
  5. Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
  6. Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
  7. Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
  8. Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
RRID
AB_2686930 (BioLegend Cat. No. 801209)
AB_2686931 (BioLegend Cat. No. 801210)

Antigen Details

Structure
Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.

Function
Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction
Alpha tubulin, kinesin and dynein.
Cell Type
Neural Stem Cells, Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin β 3 (TUBB3) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/10/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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