- 7E.17G9 (See other available formats)
- Regulatory Status
- Other Names
- Inducible COStimulatory molecule, H4
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse ICOS cDNA and ICOS hexahistidine fusion protein
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.
(PubMed link indicates BioLegend citation)
- Akbari O, et al. 2002. Nat. Med. 8:1024.
- Harada H, et al. 2003. J. Clin. Invest. 112:234.
- McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
- Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
AB_2832411 (BioLegend Cat. No. 117413)
AB_2832412 (BioLegend Cat. No. 117414)
AB_2832413 (BioLegend Cat. No. 117415)
AB_2832414 (BioLegend Cat. No. 117416)
AB_2832415 (BioLegend Cat. No. 117417)
AB_2832416 (BioLegend Cat. No. 117418)
- CD28/CTLA-4, 47-57kD
Activated T cells, subset of thymocytes
- Costimulates T cell activation, proliferation, humoral immune response
- Cell Type
- T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765
- Gene ID
- 54167 View all products for this Gene ID
- View information about CD278 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
|Biotin anti-mouse CD278 (ICOS)||7E.17G9||FC|
|PE anti-mouse CD278 (ICOS)||7E.17G9||FC|
|TotalSeq™-A0847 anti-mouse CD278 (ICOS)||7E.17G9||PG|
|TotalSeq™-C0847 anti-mouse CD278 (ICOS)||7E.17G9||PG|
|Ultra-LEAF™ Purified anti-mouse CD278 (ICOS)||7E.17G9||FC, Block|
|PE/Cyanine7 anti-mouse CD278 (ICOS)||7E.17G9||FC|
|PerCP/Cyanine5.5 anti-mouse CD278 (ICOS)||7E.17G9||FC|
|APC anti-mouse CD278 (ICOS)||7E.17G9||FC|
|TotalSeq™-B0847 anti-mouse CD278 (ICOS)||7E.17G9||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD278 (ICOS)
PE anti-mouse CD278 (ICOS)
TotalSeq™-A0847 anti-mouse CD278 (ICOS)
TotalSeq™-C0847 anti-mouse CD278 (ICOS)
Ultra-LEAF™ Purified anti-mouse CD278 (ICOS)
PE/Cyanine7 anti-mouse CD278 (ICOS)
PerCP/Cyanine5.5 anti-mouse CD278 (ICOS)
APC anti-mouse CD278 (ICOS)
TotalSeq™-B0847 anti-mouse CD278 (ICOS)