- TS2/7 (See other available formats)
- Other Names
- α1 integrin, VLA-1 α chain, Integrin α1 chain, ITGA1
- Mouse IgG1, κ
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- Product Citations
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CD49a is a 200 kD type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or Integrin α1. It associates with CD29 (β1 integrin) to form VLA-1 complex, a collagen IV and alminin-1 receptor. It is expressed on activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Human CTL line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications include: immunoprecipitation (1) and immunohistochemical staining (1) of acetone-fixed frozen tissue sections
- Additional Product Notes
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The TotalSeq™-A barcode sequence associated with clone TS2/7 is ACTGATGGACTCAGA.
The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the poly A tail, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, where B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [ACTGATGGACTCAGA]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.
(PubMed link indicates BioLegend citation)
- Hemler ME, et al. 1984. J.Immunol. 132:3011
- Hemler ME, et al. 1985. J. Biol. Chem. 260:15246
AB_2783195 (BioLegend Cat. No. 328315)
- Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex
Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells
- Adhesion, leukocyte migration
- Ligand Receptor
- With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV
- Cell Type
- Fibroblasts, Mesenchymal cells, Monocytes, NK cells, T cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122
2. Boiret N, et al. 2005. Exp. Hematol. 33:219
- Gene ID
- 3672 View all products for this Gene ID
- View information about CD49a on UniProt.org
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