Recombinant Mouse IFN-γ (carrier-free)

Pricing & Availability
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF),
Ave. Rating
2 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
575302 10 µg $45
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575304 25 µg $95
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575306 100 µg $195
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575308 500 µg $600
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Source
Mouse IFN-γ, amino acids His23-Cys155 (Accession # NM_008337) was expressed in E. coli.
Molecular Mass
The 134 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,652 Da. The DTT-reduced and non-reduced protein migrate at approximately 13 kD by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH 7.2.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The activity was measured in a cytopathic effect inhibition assay using EMC virus on L929 cells. The specific activity is 1 - 4 x 106 units/mg.
Application

Bioassay

Recommended Usage

Use when high specific biological activity is required.

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References

(PubMed link indicates BioLegend citation)
  1. Reynolds DS, et al. 1987. J. Immunol. 139:767. (BA)
  2. Dey S, et al. 2010. Mol. Cell. Biol. 30:2181. PubMed
  3. Sun T, et al. 2012. Proc. Natl. Acad. Sci. USA 109:E2117. PubMed
Product Citations
  1. Bremser A, et al. 2015. PLoS One. 10: 0137393. PubMed
  2. Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed
  3. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  4. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  5. Greer R, et al. 2016. Nat Commun. 7:13329. PubMed
  6. Inoue M, et al. 2016. Nat Neurosci. 19:1599-1609. PubMed
  7. Davis B, et al. 2017. J Virol. 10.1128/JVI.00889-17. PubMed
  8. Chu SY, et al. 2019. Nat Commun. 10:1524. PubMed
  9. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  10. Miranda K, et al. 2019. Front Immunol. 10:1049. PubMed
  11. Xiao Y, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:21. PubMed
  12. Yang L, et al. 2019. Front Immunol. 10:2020. PubMed
  13. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  14. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
Publication Library

Antigen Details

Structure
Cytokine; dimer
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 5    Revision Date: 07/01/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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