Recombinant Human CCL19 (MIP-3β) (carrier-free)

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Other Names
EBI1 ligand chemokine, ELC, macrophage inflammatory protein-3-beta
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publications
CCL19_Human_RECOM_BA_090716
BaF3-hCCR7 cells chemoattracted by human CCL19.
  • CCL19_Human_RECOM_BA_090716
    BaF3-hCCR7 cells chemoattracted by human CCL19.
Cat # Size Price Quantity Avail. Save
582102 10 µg $125
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582104 25 µg $215
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582106 100 µg $585
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582108 500 µg $1,525
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Description

CCL19 is a CC chemokine expressed in lymph nodes. CCL19 is only 32% identical to CCL21 at the amino acid level; nevertheless, these chemokines bind to the same receptor, CCR7.  CCL19 is not expressed in high endothelial venules (HEV) as CCL21, but CCL19 is transcytosed in HEV. It has been described that CCL19 binds to CCX-CKR and CRAM-B receptors. These two receptors are members of the non-classical chemokine receptor family, which also includes D6, DARC, and CXCR7. It has been suggested that CCX-CKR is a scavenging receptor for CCL19, and CRAM-B receptor might be responsible for the transcytosis in HEV for CCL19. In addition, CRAM recycles constitutively and can internalize CCL19 within a few minutes, suggesting it may play a role in the regulation of immune responses as well as homing processes.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human CCL19, amino acids (Gly22-Ser98) (Accession# NM_006274.2), was expressed in E. coli.
Molecular Mass
The 69 amino acid recombinant protein has a predicted molecular mass of approximately 8.8 kD. The DTT-reduced protein migrates at approximately 12kD and non-reduced protein at approximately 13 kD by SDS-PAGE. The N-terminal amino acid is Thr.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract baf3-hCCR7 transfectants in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Chemokine.
Distribution
CCL19 is expressed in the T cell zone of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL19 is expressed by endothelial cells in lymphatic venules.
Function
CCL19 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL19 and CCL21 inhibit apoptosis in DCs.
Interaction
Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, antigen-engaged B cells, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA).
Ligand/Receptor
CCR7 (CD197).
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Rossi DL, et al. 1997. J. Immunol. 158:1033.
2. Sánchez-Sánchez N, et al. 2004. Blood 104:619.
3. Comerford I, et al. 2006. Eur. J. Immunol. 36:1904.
4. Leick M, et al. 2009. Immunology 129:536.
5. Le Nouën C, et al. 2011. PLoS Pathog. 7:e1002105.

Gene ID
6363 View all products for this Gene ID
UniProt
View information about CCL19 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 09/07/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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