Purified anti-VGlut1 Antibody (Previously Covance catalog# MMS-5245)

Pricing & Availability
Clone
N28/9 (See other available formats)
Other Names
SLC17A7, Vesicular glutamate transporter 1, solute carrier family 17 member 7, brain-specific Na-dependent inorganic phosphate cotransporter, brain-specific Na(+)-dependent inorganic phosphate cotransporter, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Previously
Covance Catalog# MMS-5245
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_N28slash9_PURE_VGlut1_WB_110118
Western blot of purified anti-VGlut1 antibody (clone N28/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_N28slash9_PURE_VGlut1_WB_110118
    Western blot of purified anti-VGlut1 antibody (clone N28/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_N28slash9_Purified_VGlut1_Antibody_IHC_030415
    IHC staining of purified anti-VGlut1 antibody (clone N28/9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 3_N28slash9_PURE_VGlut1_1_Antibody_MB_071018
    IHC staining of purified anti-VGlut1 antibody (clone N28/9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_N28slash9_PURE_VGlut1_1_Antibody_HB_071018
    IHC staining of purified anti-VGlut1 antibody (clone N28/9) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
821302 25 µl $70.00
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821301 100 µl $140.00
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Description

Vesicular glutamate transporter (VGlut) 1, also known as solute carrier family 17 member 7 (SLC17A7) and brain-specific sodium-dependent inorganic phosphate cotransporter, is a vesicle-bound transporter of glutamate that acts in a sodium-dependent manner.  It located within the membranes of synaptic vesicles and is specifically expressed in neurons.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

RRID
AB_2734585 (BioLegend Cat. No. 821302)
AB_2564849 (BioLegend Cat. No. 821301)

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Go To Top Version: 3    Revision Date: 07/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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