Purified anti-VGlut2 Antibody (Previously Covance catalog# MMS-5206)

Pricing & Availability
Clone
N29/29 (See other available formats)
Other Names
Vesicular glutamate transporter 2, differentiation-associated BNPI, solute carrier family 17 member 6, differentation-associated Na-dependent inorganic phosphate cotransporter, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, Dnpi, Vglut2
Previously
Covance Catalog# MMS-5206
Isotype
Mouse IgG1
Ave. Rating
0 reviews
Product Citations
publications
N29slash29_Purified_VGlut2_Antibody_092717
Western blot of anti-VGlut2 antibody (clone N29/29), and isotype-matched IgG1 control. Lane 1: 20 µg of human frontal cortex membrane lysate; Lane 2: 20 µg of mouse frontal cortex membrane lysate; Lane 3: 20 µg of rat frontal cortex membrane lysate. Blots were incubated with 5 µg/mL of each primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • N29slash29_Purified_VGlut2_Antibody_092717
    Western blot of anti-VGlut2 antibody (clone N29/29), and isotype-matched IgG1 control. Lane 1: 20 µg of human frontal cortex membrane lysate; Lane 2: 20 µg of mouse frontal cortex membrane lysate; Lane 3: 20 µg of rat frontal cortex membrane lysate. Blots were incubated with 5 µg/mL of each primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • N29slash29_Purified_VGlut2_Antibody_2_071118
    Western blot of purified anti-vGlut2 antibody (clone N29/29). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate. Lane 3: 20 µg of mouse brain membrane lysate. Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-m IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426303) was used as the detection system.
  • N29slash29_Purified_VGlut2_Antibody_3_071118
    IHC staining of purified anti-vGlut2 antibody (clone N29/29) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was permeabilized with 0.25% Triton X-100 and then incubated with 10 µg/mL of the primary antibody overnight at 4°C. 2.5 µg/mL Biotinylated anti-mouse secondary antibody (Cat. No. 405303) and ABC HRP kit (Vector Labs) were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • N29slash29_Purified_VGlut2_Antibody_4_071118
    IHC staining of purified anti-VGlut2 antibody (clone N29/29) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • N29slash29_Purified_VGlut2_Antibody_5_071118
    IHC staining of purified anti-VGlut2 antibody (clone N29/29) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 555 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
832802 25 µl $70.00
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832801 100 µl $140.00
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Description

Vesicular glutamate transporter (VGlut) 2, also known as solute carrier family 17 member 6 (SLC17A6) and differentiation-associated sodium-dependent inorganic phosphate cotransporter, is expressed specifically in the brain and mediates glutamate transport at presynaptic terminals. VGlut2 is involved in sensory perception including pain and taste.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

*Expected MW: 60 kD

WB Positive Control: Rat Brain Membrane lysate

RRID
AB_2728541 (BioLegend Cat. No. 832802)
AB_2564960 (BioLegend Cat. No. 832801)

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Go To Top Version: 3    Revision Date: 07/11/2018

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