- SMI 91 (See other available formats)
- Other Names
- CNPase, Cnp-1, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase
- Mouse IgG1, κ
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- Product Citations
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High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.Product Details
- Human, Rat, Mouse
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
IHC-P - Quality tested
WB - Validated
IHC-F, ICC, EM, IP - Reported in literature
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 1.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The reactivity of Alexa Fluor® 647 anti-Myelin CNPase antibody was validated in FFPE mouse and rat brain tissue, and the HRP anti-Myelin CNPase antibody was validated in FFPE rat brain tissue.
Additional reported applications (for the relevant formats) include: immunofluorescent staining.2, 6, 8, 9
(PubMed link indicates BioLegend citation)
- Samanta J, et al. 2010. Stroke. 2:357-62. (IHC-F) PubMed
- Werner HB, et al. 2007. J. Neurosci. 27:7717-7730. (IHC-P, ICC, EM) PubMed
- Kim JY, et al. 2003. J. Neurosci. 23(13):5561-5571. (IHC-F, IP)
- Trolle C, et al. 2014. BMC Neurosci. 15:60. (IHC-F)
- Liyanage VR, et al. 2013. Mol. Autism 4:46. (ICC)
- Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
- Ding BS, et al. 2013. PLoS One 8:e62150. (EM)
- Johnson GC, et al. 2014. Pathol. 51:146. (IHC-P)
- Ferletta M, et al. 2011. Int. J. Cancer 129:45. (IHC, ICC, WB)
- Product Citations
AB_2728547 (BioLegend Cat. No. 836403)
AB_2566639 (BioLegend Cat. No. 836404)
- Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.
- Detects developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Enzymes and Regulators, Phospho-Proteins
- Gene ID
- 1267 View all products for this Gene ID
- View information about Myelin CNPase on UniProt.org
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