Purified anti-Myelin CNPase Antibody

Pricing & Availability
Clone
SMI 91 (See other available formats)
Other Names
CNPase, Cnp-1, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1-SMI91_PURE_Myelin_CNPase_MB_Antibody_042518
IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1-SMI91_PURE_Myelin_CNPase_MB_Antibody_042518
    IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2-SMI91_PURE_Myelin_CNPase_RB_Antibody_042518
    IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3-SMI91_PURE_Myelin_CNPase_Antibody_042518
    Western blot of purified anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
836403 25 µg $95
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836404 100 µg $235
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Description

High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The reactivity of Alexa Fluor® 647 anti-Myelin CNPase antibody was validated in FFPE mouse and rat brain tissue, and the HRP anti-Myelin CNPase antibody was validated in FFPE rat brain tissue.

Additional reported applications (for the relevant formats) include: immunofluorescent staining.2, 6, 8, 9

Application References

(PubMed link indicates BioLegend citation)
  1. Samanta J, et al. 2010. Stroke. 2:357-62. (IHC) PubMed
  2. Werner HB, et al. 2007. J. Neurosci. 27:7717-7730. (WB, IF) PubMed
  3. Kim JY, et al. 2003. J. Neurosci. 23(13):5561-5571. (IHC)
  4. Sprinkle TJ, et al. 1989. Crit. Rev. Neurobiol. 4:235-301.
  5. Trolle C, et al. 2014. BMC Neurosci. 15:60. (IHC)
  6. Liyanage VR, et al. 2013. Mol. Autism 4:46. (IF)
  7. Yu C, et al. 2008. Glia. 56:877. (WB)
  8. G÷ttle P, et al. 2015. J. Neurosci. 35:906. (IF)
  9. Ding BS, et al. 2013. PLoS One 8:e62150. (IF)
  10. Johnson GC, et al. 2014. Pathol. 51:146. (IHC)
  11. Ferletta M, et al. 2011. Int. J. Cancer 129:45. (WB)
Product Citations
  1. Samanta J, et al. 2010. Stroke. 41:357-362. PubMed
  2. Werner H, et al. 2007. J Neurosci. 27:7717-7730. PubMed
  3. Hill RA, et al. 2018. Nat Neurosci. 21:683. PubMed
Publication Library
RRID
AB_2728547 (BioLegend Cat. No. 836403)
AB_2566639 (BioLegend Cat. No. 836404)

Antigen Details

Structure
Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.
Interaction
Detects developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Phospho-Proteins, Enzymes and Regulators
Gene ID
1267 View all products for this Gene ID
UniProt
View information about Myelin CNPase on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/25/2018

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