Alexa Fluor® 647 anti-Myelin CNPase Antibody

Pricing & Availability
Clone
SMI 91 (See other available formats)
Other Names
CNPase, Cnp-1, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SMI-91_A647_Myelin_CNPase_Antibody_1_060618
IHC staining of Alexa Fluor® 647 anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • SMI-91_A647_Myelin_CNPase_Antibody_1_060618
    IHC staining of Alexa Fluor® 647 anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • SMI-91_A647_Myelin_CNPase_Antibody_2_060618
    IHC staining of Alexa Fluor® 647 anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • SMI-91_A647_Myelin_CNPase_Antibody_3_060618
    IHC staining of Alexa Fluor® 647 anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-91_A647_Myelin_CNPase_Antibody_4_060618
    IHC staining of Alexa Fluor® 647 anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
836407 25 µg $135
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836408 100 µg $275
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Description

High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The reactivity of Alexa Fluor® 647 anti-Myelin CNPase antibody was validated in FFPE mouse and rat brain tissue, and the HRP anti-Myelin CNPase antibody was validated in FFPE rat brain tissue.

Additional reported applications (for the relevant formats) include: immunocytochemistry staining.2, 6, 8, 9

Application References

(PubMed link indicates BioLegend citation)
  1. Samanta J, et al. 2010. Stroke. 2:357-62. (IHC) PubMed
  2. Werner HB, et al. 2007. J. Neurosci. 27:7717-7730. (WB, ICC) PubMed
  3. Kim JY, et al. 2003. J. Neurosci. 23(13):5561-5571. (IHC)
  4. Sprinkle TJ, et al. 1989. Crit. Rev. Neurobiol. 4:235-301.
  5. Trolle C, et al. 2014. BMC Neurosci. 15:60. (IHC)
  6. Liyanage VR, et al. 2013. Mol. Autism 4:46. (ICC)
  7. Yu C, et al. 2008. Glia. 56:877. (WB)
  8. Ding BS, et al. 2013. PLoS One 8:e62150. (ICC)
  9. Johnson GC, et al. 2014. Pathol. 51:146. (IHC)
  10. Ferletta M, et al. 2011. Int. J. Cancer 129:45. (WB)
RRID
AB_2734604 (BioLegend Cat. No. 836407)
AB_2734605 (BioLegend Cat. No. 836408)

Antigen Details

Structure
Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Phospho-Proteins, Enzymes and Regulators
Gene ID
1267 View all products for this Gene ID
UniProt
View information about Myelin CNPase on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/06/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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