Purified anti-NMDAR2B Antibody (Previously Covance catalog# MMS-5148)

Pricing & Availability
Clone
N59/36 (See other available formats)
Other Names
GluN2B/NR2B glutamate receptor, Glutamate receptor ionotropic, NMDA 2B, NR2B, NMDAR2B, glutamate receptor, ionotropic, NMDA2B, N-methyl D-aspartate receptor subtype 2B, glutamate [NMDA] receptor subunit epsilon-2
Previously
Covance Catalog# MMS-5148
Isotype
Mouse IgG2b
Ave. Rating
0 reviews
Product Citations
publications
N59slash36_Purified_NMDAR2B_Antibody_1_031618
Western blot of purified anti-NMDAR2B antibody (clone N59/36). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 5 µg of mouse brain membrane lysate; Lane 4: 5 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N59slash36_Purified_NMDAR2B_Antibody_1_031618
    Western blot of purified anti-NMDAR2B antibody (clone N59/36). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 5 µg of mouse brain membrane lysate; Lane 4: 5 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N59slash36_Purified_NMDAR2B_Antibody_2_031618
    IHC staining of purified anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_Purified_NMDAR2B_Antibody_3_031618
    IHC staining of purified anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_Purified_NMDAR2B_Antibody_4_031618
    IHC staining of purified anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N59slash36_Purified_NMDAR2B_Antibody_5_031618
    IHC staining of purified anti-NMDAR2B antibody (clone N59/36) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
818702 25 µl $70.00
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818701 100 µl $140.00
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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 20-271 (extracellular, N-terminus) of rat GluN2B/NR2B glutamate receptor protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg per ml. For immunohistochemistry of formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

This antibody is less reactive in human tissue in Western Blot

RRID
AB_2734578 (BioLegend Cat. No. 818702)
AB_2564823 (BioLegend Cat. No. 818701)

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Go To Top Version: 2    Revision Date: 03/16/2018

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