Purified anti-GluR2 Antibody (Previously Covance catalog# MMS-5144)

Pricing & Availability
Clone
L21/32 (See other available formats)
Other Names
GluA2/GluR2 glutamate receptor, Glutamate receptor 2, GluR2, gluR-2, glutamate receptor B, AMPA-selective glutamate receptor 2
Previously
Covance Catalog# MMS-5144
Isotype
Mouse IgG1
Ave. Rating
0 reviews
1_L21slash32_Purified_GluR2_Antibody_1_WB_020315
Western blot showing GluA2/GluR2 (L21/32) specificity. Lane 1: Molecular Weight Standard; Lane 2: Rat Brain Membrane Lysate; Lane 3: BSA negative control.
  • 1_L21slash32_Purified_GluR2_Antibody_1_WB_020315
    Western blot showing GluA2/GluR2 (L21/32) specificity. Lane 1: Molecular Weight Standard; Lane 2: Rat Brain Membrane Lysate; Lane 3: BSA negative control.
  • 2_L21slash32_Purified_GluR2_Antibody_2_IHC_112717
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
  • 3_L21slash32_Purified_GluR2_Antibody_3_IHC_112717
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
  • 4_L21slash32_Purified_GluR2_Antibody_4_IHC_112717
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
Cat # Size Price Quantity Avail. Save
810501 100 µl $140.00
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Description

Glutamate receptor 2 (GluR2), also known as glutamate receptor B, is a subunit of AMPA receptors (AMPARs) involved in fast excitatory synaptic transmission. GluR2 regulates the permeability of an AMPAR to sodium, potassium, and calcium, and the absence of GluR2 allows AMPARs to be blocked by polyamines.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 834-883 of rat GluA2/GluR2 glutamate receptor.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC-P, ICC

Recommended Usage

Each lot of this antibody is quality control tested by Western Blotting.

The optimal working dilution should be determined for each specific assay condition. 
• WB: 1.0 - 10 µg/ml
• IHC-P: 1.0 - 10 µg/ml

Tissue: Formalin-fixed Paraffin-embedded 
Pre-treatment: Citrate 
Primary incubation: 60 minutes at room temperature

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC). It was subsequently purified using protein G. It does not cross-react with GluA1/GluR1, GluA3/GluR3, or GluA4/GluR4. 

WB Positive Control: Rat Brain Membrane Lysate.

IHC-P Positive Control: Human, Mouse and Rat brain

RRID
AB_2564751 (BioLegend Cat. No. 810501)

Related FAQs

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Go To Top Version: 2    Revision Date: 06/10/2016

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