Purified anti-GluR2 Antibody (Previously Covance catalog# MMS-5144)

Pricing & Availability
Clone
L21/32 (See other available formats)
Other Names
GluA2/GluR2 glutamate receptor, Glutamate receptor 2, GluR2, gluR-2, glutamate receptor B, AMPA-selective glutamate receptor 2
Previously
Covance Catalog# MMS-5144
Isotype
Mouse IgG1
Ave. Rating
0 reviews
Product Citations
publications
1_L21slash32_Purified_GluR2_Antibody_1_WB_071818
Western blot of purified anti-GluR2 antibody (clone L21/32). Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 10 µg/mL of clone L21/32 (left) or mouse IgG1 (right) overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_L21slash32_Purified_GluR2_Antibody_1_WB_071818
    Western blot of purified anti-GluR2 antibody (clone L21/32). Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 10 µg/mL of clone L21/32 (left) or mouse IgG1 (right) overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_L21slash32_Purified_GluR2_Antibody_2_IHC_112717
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
  • 3_L21slash32_Purified_GluR2_Antibody_3_IHC_112717
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
  • 4_L21slash32_Purified_GluR2_Antibody_4_IHC_112717
    IHC staining of anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm.
Cat # Size Price Quantity Avail. Save
810502 25 µl $70.00
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810501 100 µl $140.00
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Description

Glutamate receptor 2 (GluR2), also known as glutamate receptor B, is a subunit of AMPA receptors (AMPARs) involved in fast excitatory synaptic transmission. GluR2 regulates the permeability of an AMPAR to sodium, potassium, and calcium, and the absence of GluR2 allows AMPARs to be blocked by polyamines.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 834-883 of rat GluA2/GluR2 glutamate receptor.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P). It was subsequently purified using protein G. It does not cross-react with GluA1/GluR1, GluA3/GluR3, or GluA4/GluR4.

RRID
AB_2734564 (BioLegend Cat. No. 810502)
AB_2564751 (BioLegend Cat. No. 810501)

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Go To Top Version: 3    Revision Date: 07/18/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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