Purified anti-NMDAR1 Antibody (Previously Covance catalog# MMS-5145)

Pricing & Availability
Clone
N308/48 (See other available formats)
Other Names
GluN1/NR1 glutamate receptor, Glutamate receptor ionotropic, NMDA 1, NMD-R1, neurotransmitter receptor, NMDA R1 receptor C1 cassette, N-methyl-D-aspartate glutamate receptor, glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1
Previously
Covance Catalog# MMS-5145
Isotype
Mouse IgG1
Ave. Rating
0 reviews
Product Citations
publications
1_N308slash48_PURE_NMDAR1_Antibody_WB_061418
Western blot of purified anti-NMDAR1 antibody (clone N308/48). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_N308slash48_PURE_NMDAR1_Antibody_WB_061418
    Western blot of purified anti-NMDAR1 antibody (clone N308/48). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N30848_Purified_NMDAR1_Antibody_2_030617
    IHC staining of purified anti-NMDAR1 antibody (clone N308/48) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N30848_Purified_NMDAR1_Antibody_3_030617
    IHC staining of purified anti-NMDAR1 antibody (clone N308/48) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
818602 25 µl $70.00
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818601 100 µl $140.00
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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Product Details
Technical data sheet

Product Details

Reactivity
Rat, Mouse
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 42-361 (extracellular, N-terminus) of rat GluN1/NR1 glutamate receptor protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10.0 µg per ml. For immunohistochemistry on paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

RRID
AB_2734577 (BioLegend Cat. No. 818602)
AB_2564822 (BioLegend Cat. No. 818601)

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Go To Top Version: 4    Revision Date: 06/14/2018

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