Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
XMG1.2 (See other available formats)
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
505801 50 µg $70
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505802 500 µg $115
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-?. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
  3. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  4. Kaushik DK, et al. 2019. J Clin Invest. 130. PubMed
  5. Kamdar K, et al. 2018. J Immunol. 201:230. PubMed
  6. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  7. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  8. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  9. Juneja VR, et al. 2017. J Exp Med. 214:895. PubMed
  10. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  11. Waickman AT, et al. 2017. Cytokine. 99:266. PubMed
  12. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  13. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  14. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  15. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  16. Flores–Santibáñez F, et al. 2018. Front Immunol. 0.520138889. PubMed
  17. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  18. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
  19. Knuschke T, et al. 2018. Front Immunol. 0.801388889. PubMed
  20. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  21. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  22. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  23. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  24. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  25. Asthagiri Arunkumar G, et al. 2019. Vaccine. 37:5567. PubMed
  26. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  27. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  28. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  29. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  30. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  31. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  32. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  33. Lin F, et al. 2019. Sci Adv. 5:eaax1608. PubMed
  34. Mittal D, et al. 2017. Cancer Immunol Res. 5:1098. PubMed
  35. García-Lozano A, et al. 2018. Front Microbiol. 9:1275. PubMed
  36. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  37. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
  38. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  39. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  40. Montfort M, et al. 2004. J Immunol. 173:4084. PubMed
  41. Ko S, et al. 2005. J Immunol. 175:3309. PubMed
  42. Xu G, et al. 2007. J Immunol. 179:5358. PubMed
  43. Tonkin D, et al. 2008. J Immunol. 181:4516. PubMed
  44. Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
  45. Haring J, et al. 2008. J Immunol. 180:2855. PubMed
  46. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  47. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  48. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  49. Riggan L, et al. 2020. Cell Rep. 31:107651. PubMed
  50. Uehara H, et al. 2021. eLife. 10:00. PubMed
  51. Opejin A, et al. 2020. Cell Reports. 33(8):108424. PubMed
RRID
AB_315395 (BioLegend Cat. No. 505801)
AB_315396 (BioLegend Cat. No. 505802)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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