- AN-18 (See other available formats)
- Other Names
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.Product Details
- Antibody Type
- Host Species
- Mouse IFN-γ protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
ELISPOT, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.25 - 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 3.9 to 500 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detecting antibody, and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: immunoprecipitation.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_10900246 (BioLegend Cat. No. 517901)
AB_10900809 (BioLegend Cat. No. 517902)
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
- Gene ID
- 15978 View all products for this Gene ID
- View information about IFN-gamma on UniProt.org
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