Purified anti-human CD192 (CCR2) Antibody

Pricing & Availability
Clone
K036C2 (See other available formats)
Other Names
CKR2, CCR2A, CCR2B, CKR2A, CKR2B, CMKBR2, MCP-1-R, CC-CKR-2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
K036C2_Purified_CCR2_Antibody_FC_020813
Human peripheral blood monocytes were stained with purified CCR2 (clone K036C2) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • K036C2_Purified_CCR2_Antibody_FC_020813
    Human peripheral blood monocytes were stained with purified CCR2 (clone K036C2) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
Cat # Size Price Quantity Avail. Save
357201 25 µg $95
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357202 100 µg $195
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Description

CCR2 is a chemokine receptor that binds monocyte chemoattractant proteins (MCP-1, 2, 3 and 4). Two spliced variants were initially described for CCR2 (CCR2A and CCR2B). These variants differ in their terminal carboxyl tails. Monocyte adhesion to the arterial endothelium and subsequent migration into the intima are central events in the pathogenesis of atherosclerosis. CCR2 and MCP-1 have been associated to atherosclerotic plaques. MCP-1 is induced by modified-LDL in endothelial cells and may trigger firm adhesion of monocytes to vascular endothelium under flow conditions. Local overexpression of MCP-1 at vessel walls induces infiltration of macrophages and formation of atherosclerotic lesions. Obesity induces an inflammatory state that is implicated in many clinically important complications, including insulin resistance, diabetes, atherosclerosis, and non-alcoholic fatty liver disease. CCR2 influences the development of obesity and associated adipose tissue inflammation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR2 DNA immunogen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Zhao BN, et al. 2019. J Immunol. 203:3157. PubMed
  2. Chevrier S et al. 2018. Cell systems. 6(5):612-620 . PubMed
  3. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
Publication Library
RRID
AB_2561850 (BioLegend Cat. No. 357201)
AB_2561851 (BioLegend Cat. No. 357202)

Antigen Details

Structure
Chemokine receptor, GPCR, seven transmembrane receptor
Distribution

Monocytes, macrophages, immature dendritic cells, memory T cells

Function
Induces monocyte adhesion to endothelial cells and chemotaxis
Ligand/Receptor
CCL2 (MCP-1), CCL7 (MCP-3), CCL8 (MCP-2), CCL13 (MCP-4), CCL16 (HCC4)
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Wong LM, et al. 1997. J. Biol. Chem. 272:1038.
2. Papadopoulou C, et al. 2008. Cytokine 43:181.
3. Barlic J, et al. 2007. J. Leukoc. Biol. 82:226.
4. Gu L, et al. 1998. Mol. Cell 2:275.
5. Volpe S, et al. 2012. PLoS One. 7:e37208.

Gene ID
729230 View all products for this Gene ID
UniProt
View information about CD192 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 5    Revision Date: 09/04/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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