PE anti-mouse Perforin Antibody

Pricing & Availability
Clone
S16009B (See other available formats)
Other Names
Prf1, Pfp, Pfn, Pfn-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S16009B_PE_Perforin_Antibody_1_113017
C57BL/6 splenocytes were stained with CD49b. Cells were fixed, permeabilized, and stained with PE anti-mouse Perforin (clone S16009B, top) or PE rat IgG2a, κ isotype control (bottom).
  • S16009B_PE_Perforin_Antibody_1_113017
    C57BL/6 splenocytes were stained with CD49b. Cells were fixed, permeabilized, and stained with PE anti-mouse Perforin (clone S16009B, top) or PE rat IgG2a, κ isotype control (bottom).
  • S16009B_PE_Perforin_Antibody_2_113017
See PE spectral data
Cat # Size Price Quantity Avail. Save
154405 25 µg $115
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154406 100 µg $305
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse perforin transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains.

RRID
AB_2721640 (BioLegend Cat. No. 154405)
AB_2721641 (BioLegend Cat. No. 154406)

Antigen Details

Structure
70 kD Protein that oligomerizes for pore formation.
Distribution

CTL, NK (Cytoplasmic Granules)

Function
Target cell lysis
Cell Type
T cells, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Podack ER, et al, 1988, Immunol Rev, 103:203
  2. Konjar S, et al, 2010. Immunology, 131:257
  3. Trapani JA, et al, 2002, Nat Rev Immunol, 2:735
  4. Osinska I, et al. 2014, Cent Eur J Immnol, 39:109
Gene ID
18646 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 11/30/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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