Alexa Fluor® 647 anti-human/mouse Granzyme B Antibody

Pricing & Availability
Clone
GB11 (See other available formats)
Other Names
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype
Mouse IgG1, κ
Ave. Rating
4 reviews
Product Citations
publications
1_GB11_A647_110409
Human peripheral blood lymphocytes were surface stained with CD8 PE, then intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (middle).
  • 1_GB11_A647_110409
    Human peripheral blood lymphocytes were surface stained with CD8 PE, then intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (middle).
  • 2_GB11_A647_082409
  • GB11_AF647_1_082911
    C57BL/6 mouse splenocytes were stimulated with plate-bound anti-mouse TCR-β for 2 days, then surface stained with CD8 PE and intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
515405 25 tests $145
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515406 100 tests $310
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Cross-Reactivity: Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Wever PC, et al. 1998. Immunology. 93:383
  2. Arens R, et al. 2004. J. Exp. Med. 199:1595
  3. Lima M, et al. 2003. Am. J. Pathol. 163:763
  4. Srivastava R, et al. 2015. J Immunol. 194:2232. PubMed
  5. Khan AA, et al. 2015. J Virol. 89:3776. PubMed
Product Citations
  1. Srivastava R, et al. 2015. J Immunol. 194:2232. PubMed
  2. Khan A, et al. 2015. J Virol. 89:3776. PubMed
  3. Pachnio A, et al. 2015. J Immunol. 195: 3803 - 3815. PubMed
  4. Coomes S, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.47. PubMed
  5. Kim J, et al. 2016. Sci Rep. 6:27615. PubMed
  6. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  7. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  8. Donnarumma T, et al. 2016. Cell Rep. 17:1571-1583. PubMed
  9. Griffin B, et al. 2017. Nat Commun. 10.1038/ncomms15743. PubMed
  10. Carpentier A, et al. 2017. PLoS One. 10.1371/journal.pone.0181403. PubMed
  11. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
  12. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  13. Zhang Y, et al. 2018. Nat Commun. 9:6. PubMed
  14. de Mingo Pulido Á 2018. Cancer cell. 33:60. PubMed
  15. Lin JR 2018. eLife. 7: e31657. PubMed
  16. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  17. Herndler–Brandstetter D, et al. 2018. Immunity. 48:716. PubMed
  18. Wu J, et al. 2017. Immunity. 47:1114. PubMed
  19. Miska J, et al. 2019. Cell Rep. 27:226. PubMed
Publication Library
RRID
AB_2294995 (BioLegend Cat. No. 515405)
AB_2566333 (BioLegend Cat. No. 515406)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells

Function
Induction of cell death and apoptosis
Ligand Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, B cells, Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
UniProt
View information about granzyme b on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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