Pacific Blue™ anti-human/mouse Granzyme B Antibody

Pricing & Availability
Clone
GB11 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
GB11_PB_GranzymeB_Antibody_FC_1_061013
Human peripheral blood lymphocytes were surface stained with CD8 APC and then intracellularly stained with Granzyme B (clone GB11) Pacific Blue™ (top) or mouse IgG1 Pacific Blue™ isotype control (bottom).
  • GB11_PB_GranzymeB_Antibody_FC_1_061013
    Human peripheral blood lymphocytes were surface stained with CD8 APC and then intracellularly stained with Granzyme B (clone GB11) Pacific Blue™ (top) or mouse IgG1 Pacific Blue™ isotype control (bottom).
  • GB11_PB_GranzymeB_Antibody_FC_2_061013
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
515407 25 tests $165
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515408 100 tests $358
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Wever PC, et al. 1998. Immunology. 93:383
  2. Arens R, et al. 2004. J. Exp. Med. 199:1595
  3. Lima M, et al. 2003. Am. J. Pathol. 163:763
  4. Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
  5. Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
  6. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  7. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Product Citations
  1. Khan A, et al. 2015. J Virol. 89: 6619 - 6632. PubMed
  2. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  3. Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed
  4. Burel J, et al. 2016. PLoS Negl Trop Dis. 10:e0005031. PubMed
  5. Moderzynski K, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005404. PubMed
  6. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  7. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  8. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  9. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  10. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  11. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  12. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  13. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  14. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  15. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  16. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  17. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  18. Remy-Ziller C, et al. 2017. Hum Vaccin Immunother. 10.1080/21645515.2017.1373921. PubMed
  19. Narasimhan PB, et al. 2018. PLoS Negl Trop Dis. 12:e0006404. PubMed
  20. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  21. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  22. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
  23. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  24. Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed
  25. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  26. Spolski R et al. 2019. Elife. 8 pii: e45501. PubMed
  27. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  28. Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed
  29. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  30. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  31. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  32. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  33. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
RRID
AB_2562195 (BioLegend Cat. No. 515407)
AB_2562196 (BioLegend Cat. No. 515408)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells

Function
Induction of cell death and apoptosis
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
UniProt
View information about granzyme b on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/12/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human/mouse Granzyme B

  • Alexa Fluor® 647 anti-human/mouse Granzyme B

  • Pacific Blue™ anti-human/mouse Granzyme B

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