PE anti-mouse I-Ak (Aβk) Antibody

Pricing & Availability
Clone
10-3.6 (See other available formats)
Other Names
MHC class II
Isotype
Mouse (CWB) IgG2a, κ
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Product Citations
publications
10-3.6
C3H/He mouse splenocytes stained with 10-3.6 PE
  • 10-3.6
    C3H/He mouse splenocytes stained with 10-3.6 PE
  • 10-3.6 biotin
    BALB/c mouse splenocytes stained with 10-3.6 biotin and detected with Sav-PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
109908 200 µg $265
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Description

The 10-3.6 antibody reacts with the β chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens and I-Ag7 of NOD mice; it does not react with other haplotypes (e.g., b, d, p, q).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse k haplotype, Cross-reacts with H-2 f,r,s haplotypes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g. b, d, p, q).

Application References

(PubMed link indicates BioLegend citation)
  1. Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
  2. Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
  3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
  4. Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
  5. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
  6. Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
  7. Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
  8. Busman-Sahay K, et al. 2011. J. Immunol. 186:6710. PubMed.
RRID
AB_313457 (BioLegend Cat. No. 109908)

Antigen Details

Structure
β chain of MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD4
Cell Type
B cells, T cells, Antigen-presenting cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14960 View all products for this Gene ID
UniProt
View information about I-Ak on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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