PE anti-human HLA-DRB1 Antibody

Pricing & Availability
NFLD.D2 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphoyctes were stained with CD20 APC and HLA-DRB1 (clone NFLD.D2, top) PE or mouse IgG1, κ PE isotype control (bottom).
  • NFLD.D2_PE_HLA-DRB1_Antibody_FC_1_051414.jpg
    Human peripheral blood lymphoyctes were stained with CD20 APC and HLA-DRB1 (clone NFLD.D2, top) PE or mouse IgG1, κ PE isotype control (bottom).
  • NFLD.D2_PE_HLA-DRB1_Antibody_FC_2_051414.jpg
See PE spectral data
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362303 25 tests $165
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362304 100 tests $384
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HLA-DRB1, a HLA class II beta chain paralog, is a heterodimer membrane anchored α (DRA) and β chain (DRB). It plays an imporant role in extracellular protein-derived peptide presentation. It is expressed by antigen presenting cells (APC) such as B cells, dendritic cells, and macrophages. The β chain (composed of β1 and β2) is 26-28 kD and contains polymorphisms allowing for variability in peptide binding specificities. DRB1 is expressed by all individuals and its expression is five fold higher than DRB chains 3, 4, or 5. The peptide binding groove can accommodate peptides 10-30 residues long. Thirty-three different sub-types of HLA-DRB1*04 have been described. Of these, HLA-DRB1*04 is associated with an increased incidence of rheumatoid arthritis and is present on a subset of DRB1 molecules.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
L-cell transfectants HLA-DRB1*0401(11th IHW 8115), CFA
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone NFLD.D2 recognizes a shared epitope (DRB1*70-74:QKRAA and QRRAA) of HLA_DRB1*04.

Application References

(PubMed link indicates BioLegend citation)
  1. Wicks I, et al. 1997. Ann. Rheum Dis. 56:135 (FC)
  2. Patil NS, et al. 2001. J. Immunol. 12:7157 (FC)
  3. Drover S, et al. 1998. Hum. Immunol. 59:77 (FA)
AB_2563518 (BioLegend Cat. No. 362303)
AB_2563519 (BioLegend Cat. No. 362304)

Antigen Details

MHC class II cell surface receptor, 30 kD
Presentation of the peptide to T cells leads to activation or suppression of the immune system, which eventually leads to the immune response.
CD4+ T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Scally SW, et al. 2013. J. Exp. Med. 210:2569.
2. Balnyte R, et al. 2013. BMC Neurol. 13:77.
3. Ranasinghe S, et al. 2013. Nat. Med. 19:930.
4. Foo JN, et al. 2013. Am. J. Hum. Genet. 93:167.
5. Balandraud N, et al. 2013. PLoS One. 8:e64108.
6. Sutton VR, et al. 1988. Hum. Immunol. 22:123.
7. Stunz LL, et al. 1989. J. Immunol. 143:3081.

Gene ID
3123 View all products for this Gene ID
View information about HLA-DRB1 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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