Purified anti-human HLA-DRB1 Antibody

Pricing & Availability
NFLD.D2 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphoyctes were stained with CD20 APC and purified anti-human HLA-DRB1 (clone NFLD.D2, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • NFLD_Purified_HLA-DRB1_Antibody_FC_1_061314
    Human peripheral blood lymphoyctes were stained with CD20 APC and purified anti-human HLA-DRB1 (clone NFLD.D2, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • NFLD_Purified_HLA-DRB1_Antibody_FC_2_061314
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362301 100 µg $323
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HLA-DRB1, a HLA class II beta chain paralog, is a heterodimer membrane anchored α (DRA) and β chain (DRB). It plays an imporant role in extracellular protein-derived peptide presentation. It is expressed by antigen presenting cells (APC) such as B cells, dendritic cells, and macrophages. The β chain (composed of β1 and β2) is 26-28 kD and contains polymorphisms allowing for variability in peptide binding specificities. DRB1 is expressed by all individuals and its expression is five fold higher than DRB chains 3, 4, or 5. The peptide binding groove can accommodate peptides 10-30 residues long. Thirty-three different sub-types of HLA-DRB1*04 have been described. Of these, HLA-DRB1*04 is associated with an increased incidence of rheumatoid arthritis and is present on a subset of DRB1 molecules.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
L-cell transfectants HLA-DRB1*0401(11th IHW 8115), CFA
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone NFLD.D2 recognizes a shared epitope (DRB1*70-74:QKRAA and QRRAA) of HLA_DRB1*04.

Application References

(PubMed link indicates BioLegend citation)
  1. Wicks I, et al. 1997. Ann. Rheum Dis. 56:135 (FC)
  2. Patil NS, et al. 2001. J. Immunol. 12:7157 (FC)
  3. Drover S, et al. 1998. Hum. Immunol. 59:77 (FA)
AB_2563457 (BioLegend Cat. No. 362301)

Antigen Details

MHC class II cell surface receptor, 30 kD
Presentation of the peptide to T cells leads to activation or suppression of the immune system, which eventually leads to the immune response.
CD4+ T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Scally SW, et al. 2013. J. Exp. Med. 210:2569.
2. Balnyte R, et al. 2013. BMC Neurol. 13:77.
3. Ranasinghe S, et al. 2013. Nat. Med. 19:930.
4. Foo JN, et al. 2013. Am. J. Hum. Genet. 93:167.
5. Balandraud N, et al. 2013. PLoS One. 8:e64108.
6. Sutton VR, et al. 1988. Hum. Immunol. 22:123.
7. Stunz LL, et al. 1989. J. Immunol. 143:3081.

Gene ID
3123 View all products for this Gene ID
View information about HLA-DRB1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/16/2014

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  • Purified anti-human HLA-DRB1

  • PE anti-human HLA-DRB1


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